Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
Int J Biol Macromol. 2019 Oct 1;138:744-754. doi: 10.1016/j.ijbiomac.2019.07.109. Epub 2019 Jul 18.
Ovarian cancer is the first and most important cause of malignancy death in women. Mucin 16 or MUC16 protein also known as carcinoma antigen 125 (CA 125) is the most commonly used glycoprotein for early stage diagnosis of ovarian cancer. In this work, a novel paper-based bio-device through hand writing of Ag/RGO (silver nanoparticles/reduced graphene oxide) nano-ink on the flexible paper substrate using pen-on-paper technology was developed. The prepared interface was used to the recognition of CA 125 protein in human biofluid. For this purpose, Ag/rGO nano-ink was synthesized by deposition of Ag nanoparticles onto graphene oxide sheets and the reduction of graphene oxide to rGO simultaneously. Conductivity and resistance of conductive lines were studied after drawing on photographic paper. Subsequently, to prepare a new and unique immuno-device, paper electrode modified by cysteamine caped gold nanoparticles (CysA/Au NPs) using electrochemical techniques. CysA is bonded by sulfur atoms with Au (CysA/Au NPs), and from the amine group with hydroxyl and carboxyl groups of Ag/RGO nano-ink deposited on the surface of paper-based electrodes (CysA/Au NPs/Ag-rGO). Then, anti-CA 125 antibody was immobilized on the electrode surface through Au NPs and CA 125 positively charged amine groups interaction. Atomic force microscopy, Transmission electron microscopy, Field emission scanning electron microscopy, and dynamic light scattering, were performed to identify the engineered immunosensor. Using chronoamperometry technique and under the optimized conditions, the low limit of quantitation (LLOQ) for the proposed immunoassay was recorded as 0.78 U/ml, which this evaluation was performed at highly linear range of 0.78-400 U/ml. The high sensitivity of the electrochemical immunosensor device is indicative of the ability of this immuno-device to detect early stages ovarian cancer.
卵巢癌是女性恶性肿瘤死亡的首要和最重要原因。黏蛋白 16 或 MUC16 蛋白，也称为癌抗原 125（CA 125），是用于卵巢癌早期诊断的最常用糖蛋白。在这项工作中，通过使用笔在纸上的技术在手写纸上基片上 Ag/RGO（银纳米粒子/还原氧化石墨烯）纳米墨水，开发了一种新型基于纸张的生物器件。该制备的接口用于识别人生物流体中的 CA 125 蛋白。为此，通过将银纳米粒子沉积在氧化石墨烯片上并同时将氧化石墨烯还原为 rGO 来合成 Ag/rGO 纳米墨水。研究了在照相纸上绘图后的导电线路的导电性和电阻。随后，通过电化学技术用半胱氨酸封端的金纳米粒子（CysA/Au NPs）修饰纸电极，制备了新型独特的免疫器件。CysA 通过硫原子与 Au（CysA/Au NPs）结合，并且通过胺基与 Ag/RGO 纳米墨水沉积在纸基电极表面上的羟基和羧基结合（CysA/Au NPs/Ag-rGO）。然后，通过 Au NPs 和 CA 125 带正电荷的胺基之间的相互作用将抗 CA 125 抗体固定在电极表面上。原子力显微镜、透射电子显微镜、场发射扫描电子显微镜和动态光散射用于鉴定工程化的免疫传感器。使用计时安培技术并在优化条件下，所提出的免疫测定的低定量限（LLOQ）记录为 0.78 U/ml，该评估在 0.78-400 U/ml 的高线性范围内进行。电化学免疫传感器器件的高灵敏度表明该免疫器件具有检测早期卵巢癌的能力。
