基于碳油墨/碳点/ZnO 标记的 Ag@聚吡咯复合生物标志物的电化学免疫传感用于 CA-125 卵巢癌检测。
Electrochemical immunosensing by carbon ink/carbon dot/ZnO-labeled-Ag@polypyrrole composite biomarker for CA-125 ovarian cancer detection.
机构信息
Sustainable Polymer & Innovative Composite Materials Research Group, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Road, Bangmod, Thungkru, Bangkok 10140, Thailand.
Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC; Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC; Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 10607, Taiwan, ROC.
出版信息
Bioelectrochemistry. 2023 Aug;152:108430. doi: 10.1016/j.bioelechem.2023.108430. Epub 2023 Mar 31.
In this work, we demonstrated a novel cancer antigen 125 (CA125) biomarker detection based on electrochemical immunosensor. The biomarker on conductive composite materials of carbon ink/carbon dot/zine oxide (C-ink/CD/ZnO) was employed as an electrode platform by using ITO substrate to enhance the interaction of antibodies (Ab) with supporting catalytic performance of ZnO as a labeling signal molecule. They were a scientist attention for biosensor with chemical stability, strong biocompatibility, high conductive signal, and accuracy. Moreover, the nanocomposite of silver@polypyrrole (Ag@PPy) was used as a potential redox mediator. The labeled construction with Ag@PPy was more accuracy than that of a free-labeled. The created immunosensor was a wide linear range as 1 ag·mL - 100 ng·mL and a low limitation of detection as 0.1 fg·mL under the optimal condition. This suggested that the immunosensor is considered to be an accurate and efficient diagnostic tool for CA125 and other biomarkers detection in actual sample analysis for clinic.
在这项工作中，我们展示了一种基于电化学免疫传感器的新型癌抗原 125（CA125）生物标志物检测方法。该生物标志物基于导电复合材料（碳油墨/碳点/氧化锌），使用 ITO 基底作为电极平台，以增强抗体（Ab）与支持 ZnO 作为标记信号分子的催化性能的相互作用。它们因其化学稳定性、强生物相容性、高导电信号和准确性而成为生物传感器的研究热点。此外，银@聚吡咯（Ag@PPy）纳米复合材料被用作潜在的氧化还原介体。标记结构与 Ag@PPy 的结合比游离标记更准确。在最佳条件下，所构建的免疫传感器具有较宽的线性范围（1 ag·mL-1~100 ng·mL-1）和较低的检测限（0.1 fg·mL-1）。这表明该免疫传感器有望成为 CA125 及其他生物标志物在临床实际样本分析中的准确、高效的诊断工具。
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