Department of Medical Genetics, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Gene. 2019 Sep 25;714:143955. doi: 10.1016/j.gene.2019.143955. Epub 2019 Jul 18.
MYC deregulation contributes to breast cancer development and progression. Deregulated expression levels of long non-coding RNAs (lncRNA) have been demonstrated to be critical players in development and/or maintenance of breast cancer. In this study we aimed to evaluate lncRNA expressions depending on MYC overexpression and knockdown in breast cancer cells.
Cells were infected with lentiviral vectors by either knockdown or overexpression of c-MYC. LncRNA cDNA was transcribed from total RNA samples and lncRNAs were evaluated by qRT-PCR.
Our results indicated that some of the lncRNAs having tumor suppressor (GAS5, MEG3, lincRNA-p21) and oncogenic roles (HOTAIR) are regulated by c-MYC.
We observed that c-MYC regulates lncRNAs that have important roles on proliferation, cell cycle and etc. Further studies will give us a light to identify molecular mechanisms related to MYC-lncRNA regulatory pathways in breast cancer.
MYC 失调导致乳腺癌的发生和发展。长链非编码 RNA（lncRNA）表达失调已被证明是乳腺癌发生和/或维持的关键因素。本研究旨在评估乳腺癌细胞中 MYC 过表达和敲低时 lncRNA 的表达情况。
通过慢病毒载体转染细胞，实现 c-MYC 的敲低或过表达。从总 RNA 样本中转录 lncRNA cDNA，并通过 qRT-PCR 评估 lncRNA。
我们的结果表明，一些具有肿瘤抑制（GAS5、MEG3、lincRNA-p21）和致癌作用（HOTAIR）的 lncRNAs 受 c-MYC 调控。
我们观察到 c-MYC 调节 lncRNAs，这些 lncRNAs在增殖、细胞周期等方面具有重要作用。进一步的研究将为我们提供有关 MYC-lncRNA 调控途径在乳腺癌中的分子机制的线索。
