Instituto Evandro Chagas, Seção de Arbovirologia e Febres Hemorrágicas, Ananindeua, PA, Brazil; Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
Instituto Evandro Chagas, Seção de Arbovirologia e Febres Hemorrágicas, Ananindeua, PA, Brazil.
Microb Pathog. 2019 Oct;135:103627. doi: 10.1016/j.micpath.2019.103627. Epub 2019 Jul 19.
The Guama virus (GMAV) is a member of Peribunyaviridae family, Orthobunyavirus genus. Several strains of the virus were isolated in South and Central Americas from several hosts, such as humans, wild animals, including nonhuman primates, wild rodents and mosquitoes as well as mice used as sentinels. The virus is able to cause febrile disease in humans. Here we describe for the first time pathologic and biochemical findings in golden hamsters (Mesocricetus auratus) infected with the prototype GMAV. Blood and organs of infected and control animals were collected every 24 h after infection from the 1st to the 7th day post infection (dpi) and at 21 dpi when experiment was ended. The tissues were processed for histopathology and immunohistochemistry. The blood and serum were used to determine viremia and biochemical markers plus to detect anti-GMAV antibodies. The viremia was early detected already on the 1st dpi and it was no longer detected on the 3rd dpi. Total anti-GMAV antibodies were detected from the 6th dpi. Hepatic markers as ALT of infected animals were increased and showed statistically significant difference in comparison with control animals, indicating damage of the liver; indeed the liver was the most affected organ, but other organs presented lesions and positive GMAV immunostaining as brain, lung, liver, spleen, and kidney. Our findings indicate that golden hamsters are a good animal model for experimental infection of the GMAV.
瓜马病毒（GMAV）是披膜病毒科、正布尼亚病毒属的一员。该病毒的多个毒株已在南美洲和中美洲的多种宿主中分离出来，如人类、野生动物，包括非人类灵长类动物、野生啮齿动物和蚊子以及用作哨兵的老鼠。该病毒能够引起人类发热疾病。在这里，我们首次描述了感染原型 GMAV 的金黄仓鼠（Mesocricetus auratus）的病理和生化发现。在感染后第 1 天至第 7 天（dpi）和实验结束时的第 21 天（dpi），每天收集感染和对照动物的血液和器官。对组织进行组织病理学和免疫组织化学检查。使用血液和血清来确定病毒血症和生化标志物，以及检测抗 GMAV 抗体。病毒血症早在第 1 天就被早期检测到，第 3 天就不再被检测到。从第 6 天开始检测到总抗 GMAV 抗体。感染动物的肝酶（ALT）升高，与对照动物相比差异具有统计学意义，表明肝脏受损；事实上，肝脏是受影响最严重的器官，但其他器官也出现了病变和 GMAV 免疫染色阳性，如脑、肺、肝、脾和肾。我们的研究结果表明，金黄仓鼠是 GMAV 实验感染的良好动物模型。
