CODA-CERVA, Coordination of Veterinary Diagnostics Epidemiology and Risk Analysis, Groeselenberg 99, 1180 Brussels, Belgium; CODA-CERVA, Operational Directorate Viral Diseases, Groeselenberg 99, 1180 Brussels, Belgium.
CODA-CERVA, Experimental Center, Kerklaan 62, 1830 Machelen, Belgium.
Vet Microbiol. 2014 Jun 4;170(3-4):398-402. doi: 10.1016/j.vetmic.2014.02.026. Epub 2014 Mar 3.
Schmallenberg virus (SBV) is a newly emerged virus responsible for an acute non-specific syndrome in adult cattle including high fever, decrease in milk production and severe diarrhea. It also causes reproductive problems in cattle, sheep and goat including abortions, stillbirths and malformations. The role of pigs in the epidemiology of SBV has not yet been evaluated while this could be interesting seen their suggested role in the epidemiology of the closely related Akabane virus. To address this issue, four 12 week old seronegative piglets were subcutaneously infected with 1 ml of SBV infectious serum (FLI) and kept into contact with four non-infected piglets to examine direct virus transmission. Throughout the experiment blood, swabs and feces samples were collected and upon euthanasia at 28 dpi different organs (cerebrum, cerebellum, brain stem, lung, liver, iliac lymph nodes, kidney and spleen) were sampled. No clinical impact was observed and all collected samples tested negative for SBV in rRT-PCR. Despite the absence of viremia and virus transmission, low and short lasting amounts of neutralizing antibodies were found in 2 out of 4 infected piglets. The limited impact of SBV infection in pigs was further supported by the absence of neutralizing anti-SBV antibodies in field collected sera from indoor housed domestic pigs (n=106). In conclusion, SBV infection of pigs can induce seroconversion but is ineffective in terms of virus replication and transmission indicating that pigs have no obvious role in the SBV epidemiology.
沙尔贝林病毒（SBV）是一种新出现的病毒，可导致成年牛出现急性非特异性综合征，包括高热、产奶量下降和严重腹泻。它还可导致牛、绵羊和山羊出现生殖问题，包括流产、死产和畸形。虽然猪在 SBV 流行病学中的作用尚未得到评估，但鉴于它们在密切相关的阿卡斑病毒流行病学中被认为具有的作用，这可能是很有趣的。为了解决这个问题，4 头 12 周龄的血清阴性仔猪经皮下感染 1 毫升 SBV 感染性血清（FLI），并与 4 头未感染的仔猪接触，以检查直接病毒传播。在整个实验过程中，采集了血液、拭子和粪便样本，在 28dpi 安乐死后，采集了不同的器官（大脑、小脑、脑干、肺、肝、髂淋巴结、肾和脾）样本。未观察到临床影响，所有采集的样本在 rRT-PCR 中均检测为 SBV 阴性。尽管没有病毒血症和病毒传播，但在 4 头感染仔猪中的 2 头中发现了低水平且持续时间短的中和抗体。室内饲养的家猪（n=106）采集的血清中未发现中和抗 SBV 抗体，这进一步支持了 SBV 感染对猪的影响有限。总之，猪感染 SBV 可诱导血清转化，但在病毒复制和传播方面无效，表明猪在 SBV 流行病学中没有明显作用。
