College of Biological Science, Jining Medical University, Ri'zhao, Shandong, 276800, PR China.
College of Biological Science, Jining Medical University, Ri'zhao, Shandong, 276800, PR China.
Plant Physiol Biochem. 2019 Sep;142:254-262. doi: 10.1016/j.plaphy.2019.07.017. Epub 2019 Jul 17.
The dehydration response factor (DREB) transcription factor (TF) family can function in response to multiple cues around environment in plants. Nevertheless, the functions of dehydration response factor (DREB protein) in plant cold tolerance, especially in tomatoes (Solanum lycopersicum), have been rarely studied. In this study, the functions of tomato DREB TF (SlDREB3) in cold resistance were studied using transgenic tomatoes. The level of transcripts revealed that SlDREB3 was triggered by HO and 4 °C treatments, indicating that SlDREB3 participates in response to cold stress in plants. SlDREB3-overexpressing plants exhibited high fresh mass, chlorophyll content, Fv/Fm, and O-evolving activity; low membrane damage; and reactive oxygen species accumulation under chilling stress. Furthermore, the high expression levels of late embryogenesis-abundant genes SlLEA9 and SlLEA26 were detected in transgenic plants in response to cold stress. These findings revealed that SlDREB3 overexpression improved the tolerance to cold stress in transgenic plants possibly by upregulating SlLEAs expression.
脱水反应因子（DREB）转录因子（TF）家族可以在植物中响应多种环境线索。然而，脱水反应因子（DREB 蛋白）在植物耐寒性中的功能，特别是在番茄（Solanum lycopersicum）中的功能，很少被研究。在这项研究中，使用转基因番茄研究了番茄 DREB TF（SlDREB3）在抗寒中的功能。转录本水平表明，SlDREB3 被 HO 和 4°C 处理触发，表明 SlDREB3 参与植物对冷胁迫的响应。SlDREB3 过表达植株在冷胁迫下表现出高鲜质量、叶绿素含量、Fv/Fm 和 O 演化活性；低膜损伤；和活性氧积累。此外，在冷胁迫下，转基因植株中晚期胚胎丰富基因 SlLEA9 和 SlLEA26 的表达水平升高。这些发现表明，SlDREB3 的过表达可能通过上调 SlLEAs 的表达提高了转基因植株对冷胁迫的耐受性。
