Fitzpatrick Sarah Rose, Garvey Mary, Jordan Kieran, Flynn Jim, O'Brien Bernadette, Gleeson David
Department of Livestock Systems, Teagasc, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, County Cork, Ireland.
Cellular Health and Toxicology Research Group, Institute of Technology Sligo, County Sligo, Ireland.
Vet World. 2019 May;12(5):629-637. doi: 10.14202/vetworld.2019.629-637. Epub 2019 May 6.
Teat disinfection is an important tool in reducing the incidence of bovine mastitis. Identifying the potential mastitis-causing bacterial species in milk can be the first step in choosing the correct teat disinfectant product. The objective of this study was to screen commercial teat disinfectants for inhibition against mastitis-associated bacteria isolated from various types of milk samples.
Twelve commercially available teat disinfectant products were tested, against 12 mastitis-associated bacteria strains isolated from bulk tank milk samples and bacterial strains isolated from clinical (n=2) and subclinical (n=3) quarter foremilk samples using the disk diffusion method.
There was a significant variation (7-30 mm) in bacterial inhibition between teat disinfection products, with products containing a lactic acid combination (with chlorhexidine or salicylic acid) resulting in the greatest levels of bacterial inhibition against all tested bacteria (p<0.05).
In this study, combined ingredients in teat disinfection products had greater levels of bacterial inhibition than when the ingredients were used individually. The disk diffusion assay is a suitable screening method to effectively differentiate the bacterial inhibition of different teat disinfectant products.
乳头消毒是降低奶牛乳房炎发病率的一项重要措施。识别牛奶中潜在的引起乳房炎的细菌种类可能是选择正确乳头消毒产品的第一步。本研究的目的是筛选市售乳头消毒剂对从各类牛奶样本中分离出的与乳房炎相关细菌的抑制作用。
使用纸片扩散法，对12种市售乳头消毒产品进行测试，检测其对从奶罐奶样本中分离出的12株与乳房炎相关细菌菌株以及从临床（n = 2）和亚临床（n = 3）四分之一前乳样本中分离出的细菌菌株的抑制效果。
乳头消毒产品之间的细菌抑制效果存在显著差异（7 - 30毫米），含乳酸组合（与洗必泰或水杨酸）的产品对所有测试细菌的抑制水平最高（p < 0.05）。
在本研究中，乳头消毒产品中的组合成分比单独使用各成分时具有更高的细菌抑制水平。纸片扩散试验是一种有效的筛选方法，可以有效区分不同乳头消毒产品的细菌抑制效果。
