Wolfson Institute for Biomedical Research, University College London, London, UK.
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan.
Glia. 2019 Nov;67(11):2166-2177. doi: 10.1002/glia.23684. Epub 2019 Jul 22.
Oligodendrocyte precursors (OPs) proliferate and differentiate into oligodendrocytes (OLs) during postnatal development and into adulthood in the central nervous system (CNS). Following the initiation of differentiation, OPs give rise to immature, premyelinating OLs, which undergo further differentiation and mature into myelin-forming OLs. We identified an immature OL-specific long noncoding RNA, named Pcdh17it. Through co-localization analysis and morphological characterization of OLs, we found that Pcdh17it is a specific marker for newly born immature OLs in the developing and adult forebrain of mice, and we used this new marker to analyze OL generation over the lifespan of mice. Pcdh17it is an effective tool for monitoring newly born OLs in adult brain, allowing detailed study of the dynamics of OP differentiation into OLs in the normal and pathological CNS.
少突胶质前体细胞 (OPs) 在出生后发育过程中以及中枢神经系统 (CNS) 成年期增殖并分化为少突胶质细胞 (OLs)。在分化开始后，OPs 产生未成熟的、前髓鞘形成的 OLs，这些 OLs 进一步分化并成熟为形成髓鞘的 OLs。我们鉴定了一种不成熟 OL 特异性的长非编码 RNA，命名为 Pcdh17it。通过 OL 共定位分析和形态学特征分析，我们发现 Pcdh17it 是小鼠发育中和成年大脑中新出生的不成熟 OL 的特异性标志物，我们使用这个新标志物来分析小鼠一生中 OL 的生成情况。Pcdh17it 是监测成年大脑中新出生 OL 的有效工具，允许对正常和病理 CNS 中 OP 分化为 OL 的动态进行详细研究。
