State Key Laboratory of Rare Earth Resource Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin, 130022, P. R. China.
University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.
Small. 2019 Sep;15(36):e1902522. doi: 10.1002/smll.201902522. Epub 2019 Jul 22.
The formation of bacterial biofilm is one of the causes of antimicrobial resistance, often leading to persistent infections and a high fatality rate. Therefore, there is an urgent need to develop novel and effective strategies to inhibit biofilm formation. Adenosine triphosphate (ATP) plays an important role in bacterial adhesion and biofilm formation through stimulating cell lysis and extracellular DNA (eDNA) release. Herein, a simple and robust strategy for inhibiting biofilm formation is developed using CeO -decorated porphyrin-based metal-organic frameworks (MOFs). The function of extracellular ATP (eATP) can be inhibited by CeO nanoparticles, leading to the disruption of the initial adhesion of bacteria. Furthermore, planktonic bacteria can be killed by cytotoxic reactive oxygen species (ROS) generated by MOFs. As a consequence, the synergic effect of eATP deprivation and ROS generation presents excellent capacity to prevent biofilm formation, which may provide a new direction for designing flexible and effective biofilm-inhibiting systems.
细菌生物膜的形成是导致抗菌药物耐药性的原因之一，常导致持续感染和高死亡率。因此，迫切需要开发新型有效的策略来抑制生物膜的形成。三磷酸腺苷（ATP）通过刺激细胞裂解和细胞外 DNA（eDNA）释放，在细菌黏附和生物膜形成中发挥重要作用。本文采用 CeO 修饰的卟啉基金属有机骨架（MOFs）开发了一种简单而有效的抑制生物膜形成的策略。CeO 纳米粒子可以抑制细胞外 ATP（eATP）的功能，从而破坏细菌的初始黏附。此外，MOFs 产生的细胞毒性活性氧（ROS）可以杀死浮游细菌。因此，eATP 剥夺和 ROS 生成的协同效应具有极好的抑制生物膜形成的能力，这可能为设计灵活有效的生物膜抑制系统提供了新的方向。
