Shanghai Engineering Research Center of Hierarchical Nanomaterials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Angew Chem Int Ed Engl. 2020 Dec 14;59(51):22952-22956. doi: 10.1002/anie.202008259. Epub 2020 Oct 22.
Apyrase is an important family of extracellular enzymes that catalyse the hydrolysis of high-energy phosphate bonds (HEPBs) in ATP and ADP, thereby modulating many physiological processes and driving life activities. Herein, we report an unexpected discovery that cerium-based metal-organic frameworks (Ce-MOFs) of UiO-66(Ce) have intrinsic apyrase-like activity for ATP/ADP-related physiological processes. The abundant Ce /Ce couple sites of Ce-MOFs endow them with the ability to selectively catalyse the hydrolysis of HEPBs of ATP and ADP under physiological conditions. Compared to natural enzymes, they could resist extreme pH and temperature, and present a broad range of working conditions. Based on this finding, a significant inhibitory effect on ADP-induced platelet aggregation was observed upon exposing the platelet-rich plasma (PRP) to the biomimetic UiO-66(Ce) films, prefiguring their wide application potentials in medicine and biotechnology.
磷酸三酯酶是一类重要的细胞外酶，能够催化 ATP 和 ADP 中高能磷酸键 (HEPB) 的水解，从而调节许多生理过程并驱动生命活动。在此，我们报告了一个意外的发现，即基于铈的金属有机骨架 (Ce-MOFs) 的 UiO-66(Ce) 对与 ATP/ADP 相关的生理过程具有内在的磷酸三酯酶样活性。Ce-MOFs 中丰富的 Ce3+/Ce4+ 偶联位点使它们能够在生理条件下选择性地催化 ATP 和 ADP 的 HEPB 水解。与天然酶相比，它们能够抵抗极端的 pH 值和温度，并呈现出广泛的工作条件。基于这一发现，当富含血小板的血浆 (PRP) 暴露于仿生 UiO-66(Ce) 薄膜时，观察到对 ADP 诱导的血小板聚集有显著的抑制作用，预示着它们在医学和生物技术领域的广泛应用潜力。
