Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.
Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Bialystok, Poland.
Helicobacter. 2019 Dec;24(6):e12614. doi: 10.1111/hel.12614. Epub 2019 Jul 21.
The mechanisms of downregulation of protective immunity against Helicobacter pylori (Hp) infection strongly depend on dendritic cell (DC)-induced T-lymphocyte differentiation pattern. Lactic acid bacteria (LAB) strains can modulate Hp-induced immunoresponse by changes in DC activation profiles. Here, we want to find out if the LAB-pulsed DCs will change Hp-induced T-cell responsiveness patterns.
The naive peripheral CD4 T cells were co-cultured with Hp CagA + pulsed monocyte-derived DCs (DC/CD4 T cell) in the presence/absence of the feces-derived probiotics: antagonistic or non-antagonistic to Hp (Lactobacillus rhamnosus 900, Lr, Lactobacillus paracasei 915, Lp, respectively), as assessed by the agar slab method. The regulatory T-cell (Treg) population was assessed by flow cytometry, and IFN-γ, IL-12p70, IL-10, and IL-17A levels were evaluated by ELISA method.
The Hp-pulsed DC/CD4 T-cell co-cultures were characterized by high IL-10, decreased IL-12p70 and IFN-γ levels, and elevated Treg population. In contrast, Lr-pulsed DC/CD4 T-cell co-cultures expressed low IL-10, high IL-12p70 and IFN-γ levels and declined Treg population; this responsiveness pattern was not changed by Hp. The responsiveness pattern of the Lp/Hp-pulsed DC/CD4 T-cell co-cultures did not differ from those pulsed with Hp alone.
In contrast to Lp, Lr probiotic strain overcomes Hp-mediated immune profile in the DC/T-cell co-cultures toward Th1 pattern and limited generation of Tregs in vitro. Lr may therefore be used as a component of anti-Hp treatment.
保护性免疫对幽门螺杆菌（Hp）感染的下调机制强烈依赖于树突状细胞（DC）诱导的 T 淋巴细胞分化模式。乳酸菌（LAB）菌株可以通过改变 DC 激活谱来调节 Hp 诱导的免疫反应。在这里，我们想知道 LAB 脉冲 DC 是否会改变 Hp 诱导的 T 细胞反应模式。
将幼稚外周 CD4 T 细胞与 Hp CagA+脉冲单核细胞衍生的 DC（DC/CD4 T 细胞）在存在/不存在粪便衍生的益生菌的情况下共培养：分别拮抗或非拮抗 Hp（鼠李糖乳杆菌 900，Lr，副干酪乳杆菌 915，Lp），通过琼脂平板法评估。通过流式细胞术评估调节性 T 细胞（Treg）群体，通过 ELISA 法评估 IFN-γ、IL-12p70、IL-10 和 IL-17A 水平。
Hp 脉冲 DC/CD4 T 细胞共培养的特点是高水平的 IL-10、降低的 IL-12p70 和 IFN-γ 水平以及升高的 Treg 群体。相比之下，Lr 脉冲 DC/CD4 T 细胞共培养表达低水平的 IL-10、高水平的 IL-12p70 和 IFN-γ 以及降低的 Treg 群体；这种反应模式不受 Hp 的影响。与单独用 Hp 脉冲相比，Lp/Hp 脉冲 DC/CD4 T 细胞共培养的反应模式没有差异。
与 Lp 不同，Lr 益生菌菌株在体外克服了 DC/T 细胞共培养中 Hp 介导的免疫谱向 Th1 模式和 Treg 生成受限的方向。因此，Lr 可作为抗 Hp 治疗的一部分。
