Institute of Molecular Medicine, Renji Hospital, School of Medicine , Shanghai Jiao Tong University , Shanghai , 200127 , China.
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-sensing and Chemometrics, College of Chemistry and Chemical Engineering , Hunan University , Changsha , 410082 , China.
J Am Chem Soc. 2019 Aug 14;141(32):12738-12743. doi: 10.1021/jacs.9b05470. Epub 2019 Aug 1.
The ability to accurately identify and isolate cells is the cornerstone of precise disease diagnosis and therapies. A single-step cell identification method based on logic analysis of multiple surface markers will have unique advantages because of its accuracy and efficacy. Herein, using multiple DNA aptamers for cancer biomarker recognition and associative toehold activation for signal integration and amplification as two molecular keys, we have successfully operated a cell-surface device that can perform AND Boolean logic analysis of multiple biomarkers and precisely label the target cell subtype in large populations of similar cells via the presence or absence of different biomarkers. Our approach can achieve single-step cancer cell identification and isolation with excellent sensitivity and accuracy and thus will have broad applications in biological science, biomedical engineering, and personalized medicine.
准确识别和分离细胞是精准疾病诊断和治疗的基石。基于对多个表面标志物的逻辑分析的一步式细胞识别方法，由于其准确性和功效，将具有独特的优势。在这里，我们使用多个 DNA 适体来识别癌症生物标志物，并将关联的引发结构用于信号整合和放大作为两个分子关键，成功地操作了一种细胞表面设备，该设备可以对多个生物标志物进行 AND 布尔逻辑分析，并通过存在或不存在不同的生物标志物来精确标记类似细胞群体中的目标细胞亚型。我们的方法可以实现一步式癌细胞识别和分离，具有出色的灵敏度和准确性，因此将在生物科学、生物医学工程和个性化医疗中有广泛的应用。
