Ozlu Busra, Ergin Mert, Budak Sevcan, Tunali Selcuk, Yildirim Nuh, Erisken Cevat
Department of Biomedical Engineering, TOBB University of Economics and Technology, Ankara, Turkey.
School of Medicine, TOBB University of Economics and Technology, Ankara, Turkey.
Int J Artif Organs. 2019 Dec;42(12):757-764. doi: 10.1177/0391398819863434. Epub 2019 Jul 21.
Despite remarkable advancement in the past decades, heart-related defects are still prone to progress irreversibly and can eventually lead to heart failure. A personalized extracellular matrix-based bioartificial heart created by allografts/xenografts emerges as an alternative as it can retain the original three-dimensional architecture combined with a preserved natural heart extracellular matrix. This study aimed at developing a procedure for decellularizing heart tissue harvested from rats and evaluating decellularization efficiency in terms of residual nuclear content and structural properties. Tissue sections showed no or little visible cell nuclei in decellularized heart, whereas the native heart showed dense cellularity. In addition, there was no significant variation in the alignment of muscle fibers upon decellularization. Furthermore, no significant difference was detected between native and decellularized hearts in terms of fiber diameter. Our findings demonstrate that fiber alignment and diameter can serve as additional parameters in the characterization of biological heart scaffolds as these provide valuable input for evaluating structural preservation of decellularized heart. The bioartificial scaffold formed here can be functionalized with patient's own material and utilized in regenerative engineering.
尽管在过去几十年里取得了显著进展，但心脏相关缺陷仍易于发生不可逆的进展，并最终可能导致心力衰竭。由同种异体移植物/异种移植物创建的基于个性化细胞外基质的生物人工心脏成为一种替代方案，因为它可以保留原始的三维结构，并结合保留的天然心脏细胞外基质。本研究旨在开发一种从大鼠获取的心脏组织去细胞化程序，并从残留核含量和结构特性方面评估去细胞化效率。组织切片显示，去细胞化心脏中没有或几乎没有可见的细胞核，而天然心脏显示出密集的细胞性。此外，去细胞化后肌纤维的排列没有显著变化。此外，在纤维直径方面，天然心脏和去细胞化心脏之间未检测到显著差异。我们的研究结果表明，纤维排列和直径可作为表征生物心脏支架的额外参数，因为这些参数为评估去细胞化心脏的结构保存提供了有价值的信息。这里形成的生物人工支架可以用患者自身的材料进行功能化，并用于再生工程。
