Jiao Xin, Wang Zengguang, Li Yiming, Wang Tianchang, Xu Chen, Zhou Xianhao, Gan Yaokai
Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Bioeng Biotechnol. 2023 Mar 30;11:1171360. doi: 10.3389/fbioe.2023.1171360. eCollection 2023.
Tendinopathy is a common disease in orthopaedics, seriously affecting tendon functions. However, the effects of non-surgical treatment on tendinopathy are not satisfactory and surgical treatments possibly impair the function of tendons. Biomaterial fullerenol has been proved to show good anti-inflammatory effects on various inflammatory diseases. For experiments, primary rat tendon cells (TCs) were treated by interleukin-1 beta (IL-1β) combined with aqueous fullerenol (5, 1, 0.3 μg/mL). Then inflammatory factors, tendon-related markers, migration and signaling pathways were detected. For experiments, rat tendinopathy model was constructed by local injection of collagenase into Achilles tendons of rats and fullerenol (0.5, 1 mg/mL) was locally injected 7 days after collagenase injection. Inflammatory factors and tendon-related markers were also investigated. Fullerenol with good water-solubility showed excellent biocompatibility with TCs. Fullerenol could increase expression of tendon-related factors (Collagen I and tenascin C) and decrease expression of inflammatory factors (matrix metalloproteinases-3, MMP-3, and MMP-13) and reactive oxygen species (ROS) level. Simultaneously, fullerenol slowed the migration of TCs and inhibited activation of Mitogen-activated protein kinase (MAPK) signaling pathway. Fullerenol also attenuated tendinopathy , including reduction of fiber disorders, decrease of inflammatory factors and increase of tendon markers. In summary, fullerenol is a promising biomaterial that can be used to treat tendinopathy.
肌腱病是骨科的一种常见疾病，严重影响肌腱功能。然而，非手术治疗对肌腱病的效果并不理想，而手术治疗可能会损害肌腱功能。生物材料富勒醇已被证明对各种炎症性疾病具有良好的抗炎作用。在实验中，用白细胞介素-1β（IL-1β）联合富勒醇水溶液（5、1、0.3μg/mL）处理原代大鼠肌腱细胞（TCs）。然后检测炎症因子、肌腱相关标志物、迁移和信号通路。在实验中，通过向大鼠跟腱局部注射胶原酶构建大鼠肌腱病模型，并在注射胶原酶7天后局部注射富勒醇（0.5、1mg/mL）。还研究了炎症因子和肌腱相关标志物。具有良好水溶性的富勒醇与TCs表现出优异的生物相容性。富勒醇可增加肌腱相关因子（I型胶原和肌腱蛋白C）的表达，降低炎症因子（基质金属蛋白酶-3、MMP-3和MMP-13）的表达和活性氧（ROS）水平。同时，富勒醇减缓了TCs的迁移并抑制了丝裂原活化蛋白激酶（MAPK）信号通路的激活。富勒醇还减轻了肌腱病，包括减少纤维紊乱（纤维排列紊乱）、降低炎症因子水平和增加肌腱标志物。总之，富勒醇是一种有前景的可用于治疗肌腱病的生物材料。
