Department of Gastroenterology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, China.
Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China.
J Clin Lab Anal. 2019 Nov;33(9):e22984. doi: 10.1002/jcla.22984. Epub 2019 Jul 22.
Circular RNAs (circRNAs) are a special class of endogenous noncoding RNAs that have numerous biological functions in normal situation and diseases including cancers. However, the clinical significance of circRNAs in gastric cancer (GC) remains largely unknown. Here, we chose two representative circRNAs, hsa_circ_0067582 and hsa_circ_0005758, to investigate their clinical significance in GC patients.
Using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we explored the expression levels of hsa_circ_0067582 and hsa_circ_0005758 in tissues with different stages of gastric tumorigenesis. Then, the relationships between their expression levels and GC patients' clinicopathological factors were further investigated. Receiver operating characteristic (ROC) curves were established for evaluating diagnostic values of hsa_circ_0067582 and hsa_circ_0005758.
Compared with healthy control tissues, both hsa_circ_0067582 and hsa_circ_0005758 were significantly decreased in GC tissues. Besides, hsa_circ_0067582 expression was associated with GC patients' tissue CEA level (P <.001) and stages (P = .037); and hsa_circ_0005758 expression was relevant to tissue CEA level (P < .001) and perineural invasion (P = .048). The area under the ROC curve (AUC) of hsa_circ_0067582 was up to 0.671. The cutoff value was set at 10.61, with which the sensitivity and specificity were 55.2% and 75.0%, respectively. Similar to hsa_circ_0005758, the AUC of hsa_circ_0005758 was 0.721. The cutoff value was set at 10.20, with which the sensitivity and specificity were 75.0% and 67.7%, respectively.
These results showed that both hsa_circ_0067582 and hsa_circ_0005758 may play an important role in gastric carcinogenesis; and they may be potential indicators for GC diagnosis.
环状 RNA（circRNAs）是一类特殊的内源性非编码 RNA，在正常情况下和包括癌症在内的疾病中具有多种生物学功能。然而，circRNAs 在胃癌（GC）中的临床意义在很大程度上仍然未知。在这里，我们选择了两个有代表性的 circRNAs，hsa_circ_0067582 和 hsa_circ_0005758，来研究它们在 GC 患者中的临床意义。
使用实时定量逆转录聚合酶链反应（qRT-PCR），我们检测了不同阶段胃肿瘤发生组织中 hsa_circ_0067582 和 hsa_circ_0005758 的表达水平。然后，进一步研究了它们的表达水平与 GC 患者临床病理因素之间的关系。建立了接收器工作特征（ROC）曲线，以评估 hsa_circ_0067582 和 hsa_circ_0005758 的诊断价值。
与健康对照组织相比，GC 组织中 hsa_circ_0067582 和 hsa_circ_0005758 的表达均显著降低。此外，hsa_circ_0067582 的表达与 GC 患者组织 CEA 水平（P <.001）和分期（P =.037）相关；而 hsa_circ_0005758 的表达与组织 CEA 水平（P <.001）和神经周围浸润（P =.048）相关。hsa_circ_0067582 的 ROC 曲线下面积（AUC）高达 0.671。将截断值设定为 10.61，其敏感性和特异性分别为 55.2%和 75.0%。类似地，hsa_circ_0005758 的 AUC 为 0.721。将截断值设定为 10.20，其敏感性和特异性分别为 75.0%和 67.7%。
这些结果表明，hsa_circ_0067582 和 hsa_circ_0005758 可能在胃癌发生中发挥重要作用；它们可能是 GC 诊断的潜在指标。
