Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
Fuyang Fifth People's Hospital, Fuyang, China.
J Clin Lab Anal. 2021 Feb;35(2):e23642. doi: 10.1002/jcla.23642. Epub 2020 Nov 7.
As far as gastric cancer is concerned, there is a lack of specific early diagnostic biomarkers in clinical diagnosis. Circular RNAs (circRNAs) have been found to be stable in gastric cancer tissues and plasma, so they have the potential to become new type of diagnostic biomarkers for gastric cancer.
The hsa_circ_0001811 expressions in gastric cancer tissues and paired non-cancer tissues, preoperative and postoperative plasma of patients with gastric cancer, and plasma of healthy volunteers were detected using quantitative reverse transcription-polymerase chain reaction. The receiver operating characteristic (ROC) curves were drawn; and the combined ROC curves were used to analyze their diagnostic values. The correlation between the plasma or tissue hsa_circ_0001811 levels and the clinicopathological factors of gastric cancer was further analyzed.
Hsa_circ_0001811 was found to be lowly expressed in gastric cancer tissues and plasma from patients with gastric cancer. The area under the ROC curve (AUC) in the gastric cancer tissues and the plasma of patients with gastric cancer was 0.658 and 0.747, respectively. The AUC increased to 0.824 after combination of both. The expression level of hsa_circ_0001811 in gastric cancer tissue correlated with carcinoembryonic antigen (CEA) (P = .0347), tissue differentiation (P = .0138), and lymph node metastasis (P = .0234), while plasma hsa_circ_0001811 level was related to carbohydrate antigen (CA19-9) (P = .0278), lymph node metastasis (P = .0469), distant metastasis (P = .0384), and age (P = .0085).
The above results indicate that hsa_circ_0001811 may become a new biomarker for clinical diagnosis of gastric cancer.
就胃癌而言，临床诊断中缺乏特异性的早期诊断生物标志物。环状 RNA（circRNAs）已在胃癌组织和血浆中被发现稳定存在，因此它们有可能成为胃癌的新型诊断生物标志物。
采用实时定量逆转录聚合酶链反应检测胃癌组织及其配对非癌组织、胃癌患者术前和术后血浆以及健康志愿者血浆中的 hsa_circ_0001811 表达。绘制受试者工作特征（ROC）曲线；并采用联合 ROC 曲线分析其诊断价值。进一步分析血浆或组织 hsa_circ_0001811 水平与胃癌临床病理因素的相关性。
hsa_circ_0001811 在胃癌组织和胃癌患者血浆中表达水平较低。胃癌组织和胃癌患者血浆的 ROC 曲线下面积（AUC）分别为 0.658 和 0.747，两者联合后 AUC 增加至 0.824。hsa_circ_0001811 在胃癌组织中的表达水平与癌胚抗原（CEA）（P=0.0347）、组织分化（P=0.0138）和淋巴结转移（P=0.0234）相关，而血浆 hsa_circ_0001811 水平与碳水化合物抗原（CA19-9）（P=0.0278）、淋巴结转移（P=0.0469）、远处转移（P=0.0384）和年龄（P=0.0085）相关。
上述结果表明，hsa_circ_0001811 可能成为胃癌临床诊断的新型生物标志物。
