1Department of Pharmacology II, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
2Department of Clinical Pharmacology and Therapeutics, Faculty of Medicine, Oita University, Yufu, Japan.
J Med Food. 2019 Aug;22(8):789-796. doi: 10.1089/jmf.2018.4340. Epub 2019 Jul 18.
Royal jelly (RJ) is widely used as a cosmetic or dietary supplement to relieve various health disorders, such as dry skin, fatigue, and menopause. RJ has been recommended to improve constipation on a commercial basis. However, the detailed mechanisms by which RJ influences intestinal motility and whether RJ improves constipation remain unclear. Therefore, we investigated the effects of RJ on the motility of mouse ileum both and . Using myograph methods, RJ dose-dependently induced contractions of isolated ileal segments, which were inhibited by treatment with atropine. Eserine sulfate, a cholinesterase inhibitor, enhanced the RJ-induced contractions, whereas RJ treated with acetylcholinesterase did not result in ileum contraction. RJ-induced contractions were not affected by -nitro-l-arginine methyl ester, a nitric oxide synthase inhibitor, although nicotine-induced contractions were significantly enhanced. In contrast, in a gastrointestinal (GI) transit model, single oral administration of 300 mg/kg RJ did not affect GI transit in both normal mice and the loperamide-induced constipation model mice. These results demonstrate that acetylcholine in RJ directly acted on the muscarinic receptors of the mouse intestinal smooth muscle, causing it to contract . In contrast, single oral administration of RJ did not improve constipation. This study is the first to evaluate the effects of RJ on the motility of mouse ileum in and experiments for the validation of application of RJ as a gentle laxative.
蜂王浆（RJ）被广泛用作化妆品或膳食补充剂，以缓解各种健康障碍，如皮肤干燥、疲劳和更年期。RJ 已被推荐用于改善便秘的商业基础上。然而，RJ 影响肠道动力的详细机制以及 RJ 是否改善便秘仍然不清楚。因此，我们研究了 RJ 对小鼠回肠运动的影响。使用肌动描记法，RJ 剂量依赖性地诱导离体回肠段收缩，用阿托品处理可抑制该收缩。乙酰胆碱酯酶抑制剂硫酸依色林增强了 RJ 诱导的收缩，而用乙酰胆碱酯酶处理的 RJ 则不会导致回肠收缩。RJ 诱导的收缩不受一氧化氮合酶抑制剂 - 硝基 -l-精氨酸甲酯的影响，尽管烟碱诱导的收缩明显增强。相比之下，在胃肠道（GI）转运模型中，单次口服 300mg/kg RJ 对正常小鼠和洛哌丁胺诱导的便秘模型小鼠的 GI 转运均无影响。这些结果表明，RJ 中的乙酰胆碱直接作用于小鼠肠平滑肌的毒蕈碱受体，导致其收缩。相比之下，RJ 的单次口服给药并没有改善便秘。这项研究首次评估了 RJ 在和实验中对小鼠回肠运动的影响，以验证 RJ 作为温和泻药的应用。
