芳樟醇调节皮肤癣菌对唑类药物的敏感性。

Linalool modulates dermatophyte susceptibility to azole drugs.

作者信息

Ponte Hellen Aparecida Silva, Lima Maria Islaine de Oliveira, Lima Edeltrudes de Oliveira, Pereira Fillipe de Oliveira

机构信息

Laboratory of Biochemistry, Academic Unit of Health, Education and Health Center, Federal University of Campina Grande, Cuité, Brazil.

Laboratory of Mycology, Department of Pharmaceutical Science, Health Sciences Center, Federal University of Paraıba, João Pessoa, Brazil.

出版信息

Med Mycol. 2020 Feb 1;58(2):272-274. doi: 10.1093/mmy/myz041.

DOI:10.1093/mmy/myz041

PMID:31329906

Abstract

This study investigated the monoterpene linalool and its resistance modulating activity involving ergosterol biosynthesis inhibitors (ketoconazole, fluconazole, and itraconazole) in strains of Microsporum spp. and Trichophyton spp. The minimum inhibitory concentration (MIC) of test-drugs were determined by microdilution. The modulating effect of linalool was evaluated by determining the MIC of the antifungals in the presence of subinhibitory concentrations of linalool. We also investigated the association effect (checkerboard) of linalool together with ketoconazole and itraconazole. The fungi became more sensitive to ketoconazole and itraconazole in the presence of linalool. The linalool and azole drug associations presented synergism.

摘要

本研究调查了单萜芳樟醇及其在微孢子菌属和毛癣菌属菌株中涉及麦角甾醇生物合成抑制剂（酮康唑、氟康唑和伊曲康唑）的耐药调节活性。通过微量稀释法测定受试药物的最低抑菌浓度（MIC）。通过在亚抑菌浓度的芳樟醇存在下测定抗真菌药物的MIC来评估芳樟醇的调节作用。我们还研究了芳樟醇与酮康唑和伊曲康唑的联合效应（棋盘法）。在芳樟醇存在下，真菌对酮康唑和伊曲康唑变得更敏感。芳樟醇与唑类药物联合呈现协同作用。