Department Public Health and Pediatrics, Microbiology Division, University of Turin, 10126 Turin, Italy.
Area Servizi Alla Ricerca Polo Agraria e Medicina Veterinaria, University of Turin, 10095 Turin, Italy.
Molecules. 2019 Aug 29;24(17):3148. doi: 10.3390/molecules24173148.
The promising antimicrobial activity of essential oils (EOs) has led researchers to use them in combination with antimicrobial drugs in order to reduce drug toxicity, side effects, and resistance to single agents. x , known worldwide as "Mentha of Pancalieri", is produced locally at Pancalieri (Turin, Italy). The EO from this species is considered as one of the best mint EOs in the world. In our research, we assessed the antifungal activity of "Mentha of Pancalieri" EO, either alone or in combination with azole drugs (fluconazole, itraconazole, ketoconazole) against a wide panel of yeast and dermatophyte clinical isolates. The EO was analyzed by GC-MS, and its antifungal properties were evaluated by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) parameters, in accordance with the CLSI guidelines, with some modifications. The interaction of EO with azoles was evaluated through the chequerboard and isobologram methods. The results suggest that this EO exerts a fungicidal activity against yeasts and a fungistatic activity against dermatophytes. Interaction studies with azoles indicated mainly synergistic profiles between itraconazole and EO vs. spp., and . Thus, the "Mentha of Pancalieri" EO may act as a potential antifungal agent and could serve as a natural adjuvant for fungal infection treatment.
植物精油（EOs）具有良好的抗菌活性，这促使研究人员将其与抗菌药物联合使用，以降低药物毒性、副作用和单一药物的耐药性。香薄荷，又名“潘卡列里薄荷”，在意大利都灵的潘卡列里本地生产。这种薄荷精油被认为是世界上最好的薄荷精油之一。在我们的研究中，我们评估了“潘卡列里薄荷”精油单独或与唑类药物（氟康唑、伊曲康唑、酮康唑）联合使用对一系列酵母和皮肤真菌临床分离株的抗真菌活性。按照 CLSI 指南，通过 GC-MS 对精油进行了分析，并通过最低抑菌浓度（MIC）和最低杀菌浓度（MFC）参数评估了其抗真菌特性，对某些参数进行了修改。通过棋盘和等浓度图方法评估了精油与唑类药物的相互作用。结果表明，该精油对酵母具有杀菌活性，对皮肤真菌具有抑菌活性。与唑类药物的相互作用研究表明，酮康唑与 EO 之间主要为协同作用，而酮康唑与 spp.、 和 之间为协同作用。因此，“潘卡列里薄荷”精油可能作为一种潜在的抗真菌剂，并可作为治疗真菌感染的天然佐剂。
