Programa Moscafrut, SADER/SENASICA-IICA, Metapa de Domínguez, Chiapas, México.
Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Seibersdorf, Vienna, Austria.
J Econ Entomol. 2019 Aug 3;112(4):1604-1610. doi: 10.1093/jee/toz094.
For many years, an area-wide fruit fly control campaign against the Mexican fruit fly, Anastrepha ludens (Loew) has been implemented in some regions of Mexico and Texas, using the sterile insect technique (SIT) as its principal component. To improve the efficiency of the SIT, a genetic sexing strain based on black pupae mutation (bp) was developed for A. ludens, namely, 'Tapachula-7' (Tap-7 genetic sexing strains [GSSs]). This strain was introduced into the AW-IPM program recently and allows male-only releases for SIT applications. Here, we report the genetic and biological characterization of a new mutation, slow larvae (sl), which was introduced to the original translocation of the Tap-7 GSS resulting in two new GSS (slow-7 and Tap/slow-7). In both GSSs, the translocated wild-type males emerge from brown pupae that develop faster than females. The females are homozygous for sl mutation in the slow-7 GSS and homozygous for sl and bp mutations in the Tap/slow-7 GSS, reaching larval maturity 2 d after most of the wild-type males, allowing the separation of most males during pupariation. The potential use of the slow-7 and Tap/slow-7 GSSs in mass rearing and large-scale population suppression programs is discussed.
多年来，墨西哥和德克萨斯州的一些地区一直在实施针对墨西哥果蝇（Anastrepha ludens (Loew)）的区域范围的果蝇控制运动，该运动主要采用不育昆虫技术（SIT）。为了提高 SIT 的效率，针对 A. ludens 开发了一种基于黑蛹突变（bp）的遗传性别鉴定株系（GSS），即“塔帕楚拉-7”（Tap-7 遗传性别鉴定株系 [GSS]）。该株系最近被引入 AW-IPM 计划，允许仅释放雄性用于 SIT 应用。在这里，我们报告了一种新突变（slow larvae [sl]）的遗传和生物学特征，该突变被引入到 Tap-7 GSS 的原始易位中，导致了两个新的 GSS（slow-7 和 Tap/slow-7）。在这两个 GSS 中，易位的野生型雄性从发育速度比雌性快的棕色蛹中出现。在 slow-7 GSS 中，雌性是 sl 突变的纯合子，而在 Tap/slow-7 GSS 中是 sl 和 bp 突变的纯合子，在大多数野生型雄性之后 2 天达到幼虫成熟，从而在蛹化期间分离出大多数雄性。讨论了 slow-7 和 Tap/slow-7 GSS 在大规模繁殖和大规模种群抑制计划中的潜在用途。
