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基于壳寡糖、ε-聚赖氨酸和 spp. 次级代谢产物的抗真菌剂对三种葡萄座腔菌科物种的作用

Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and spp. Secondary Metabolites against Three Botryosphaeriaceae Species.

作者信息

Buzón-Durán Laura, Martín-Gil Jesús, Pérez-Lebeña Eduardo, Ruano-Rosa David, Revuelta José L, Casanova-Gascón José, Ramos-Sánchez M Carmen, Martín-Ramos Pablo

机构信息

Departamento de Ingeniería Agroforestal, ETSIIAA, Universidad de Valladolid, Avenida de Madrid 44, 34004 Palencia, Spain.

Instituto Tecnológico Agrario de Castilla y León, Unidad de Cultivos Leñosos y Hortícolas, Ctra. De Burgos km 119, Finca Zamadueñas, 47071 Valladolid, Spain.

出版信息

Antibiotics (Basel). 2019 Jul 20;8(3):99. doi: 10.3390/antibiotics8030099.

DOI:10.3390/antibiotics8030099
PMID:31330856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6783921/
Abstract

Grapevine trunk diseases (GTDs) are a major threat to the wine and grape industry. The aim of the study was to investigate the antifungal activity against , , and of ε-polylysine, chitosan oligomers, their conjugates, and culture filtrates, and their binary mixtures with chitosan oligomers. In vitro mycelial growth inhibition tests suggest that the efficacy of these treatments, in particular those based on ε-polylysine and ε-polylysine:chitosan oligomers 1:1 / conjugate, against the three Botryosphaeriaceae species would be comparable to or higher than that of conventional synthetic fungicides. In the case of ε-polylysine, EC values as low as 227, 26.9, and 22.5 µg·mL were obtained for , , and , respectively. Although the efficacy of the conjugate was slightly lower, with EC values of 507.5, 580.2, and 497.4 µg·mL, respectively, it may represent a more cost-effective option to the utilization of pure ε-polylysine. The proposed treatments may offer a viable and sustainable alternative for controlling GTDs.

摘要

葡萄树干病害(GTDs)是葡萄酒和葡萄产业的重大威胁。本研究的目的是调查ε-聚赖氨酸、壳寡糖、它们的共轭物、[未提及物质1]和[未提及物质2]培养滤液以及它们与壳寡糖的二元混合物对[未提及的三种真菌]的抗真菌活性。体外菌丝生长抑制试验表明,这些处理方法,特别是基于ε-聚赖氨酸和ε-聚赖氨酸:壳寡糖1:1/共轭物的处理方法,对三种葡萄座腔菌属菌种的效果与传统合成杀菌剂相当或更高。就ε-聚赖氨酸而言,对[未提及的三种真菌]分别获得了低至227、26.9和22.5 µg·mL的EC值。虽然共轭物的效果略低,EC值分别为507.5、580.2和497.4 µg·mL,但它可能是使用纯ε-聚赖氨酸更具成本效益的选择。所提出的处理方法可能为控制葡萄树干病害提供一种可行且可持续的替代方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/37428cfd70ab/antibiotics-08-00099-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/75e437d11936/antibiotics-08-00099-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/ec4b0bbf198c/antibiotics-08-00099-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/37428cfd70ab/antibiotics-08-00099-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/75e437d11936/antibiotics-08-00099-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/ec4b0bbf198c/antibiotics-08-00099-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0de0/6783921/37428cfd70ab/antibiotics-08-00099-g003.jpg

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