GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany.
Faculty of Mathematics and Natural Sciences, Kiel University, Christian-Albrechts-Platz 4, 24118 Kiel, Germany.
Mar Drugs. 2019 Jul 19;17(7):419. doi: 10.3390/md17070419.
(eelgrass) is a marine foundation species with key ecological roles in coastal habitats. Its bacterial microbiota has been well studied, but very little is known about its mycobiome. In this study, we have isolated and identified 13 fungal strains, dominated by species (10 strains), from the leaf and the root rhizosphere of Baltic . The organic extracts of the fungi that were cultured by an OSMAC (One-Strain-Many-Compounds) regime using five liquid culture media under both static and shaking conditions were investigated for their chemical and bioactivity profiles. All extracts showed strong anti-quorum sensing activity, and the majority of the extracts displayed antimicrobial or anti-biofilm activity against Gram-negative environmental marine and human pathogens. HPLC-DAD-MS-based rapid metabolome analyses of the extracts indicated the high influence of culture conditions on the secondary metabolite (SM) profiles. Among 69 compounds detected in all sp. extracts, 46 were successfully dereplicated. Analysis of SM relatedness in culture conditions by Hierarchical Cluster Analysis (HCA) revealed generally low similarity and showed a strong effect of medium selection on chemical profiles of sp. This is the first study assessing both the metabolite and bioactivity profile of the fungi associated with Baltic eelgrass .
（鳗草）是一种海洋基础物种，在沿海生境中具有关键的生态作用。它的细菌微生物群已经得到了很好的研究，但对其真菌组知之甚少。在这项研究中，我们从波罗的海鳗草的叶片和根际中分离并鉴定了 13 株真菌，其中以 种（10 株）为主。我们采用 OSMAC（一种化合物多种培养）方法，使用五种液体培养基在静态和振荡条件下培养真菌，研究了它们的化学和生物活性谱。所有提取物都表现出强烈的抗群体感应活性，而且大多数 提取物对革兰氏阴性环境海洋和人类病原体具有抗菌或抗生物膜活性。基于 HPLC-DAD-MS 的快速代谢组分析表明，培养条件对次生代谢物（SM）谱有很大影响。在所有 种提取物中检测到的 69 种化合物中，有 46 种成功地被鉴定。通过层次聚类分析（HCA）对培养条件下 SM 相关性的分析表明，一般来说，相似性较低，而且培养基的选择对 种的化学谱有很强的影响。这是首次评估与波罗的海鳗草相关的真菌的代谢物和生物活性谱。
