效应性 CD4 T 细胞的 IL-22 产生在 SLE 患者中发生改变。
IL-22 production of effector CD4 T-cells is altered in SLE patients.
机构信息
Department of Infectious Diseases, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
Department of Nephrology, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
出版信息
Eur J Med Res. 2019 Jul 22;24(1):24. doi: 10.1186/s40001-019-0385-6.
BACKGROUND
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by T-cell-dependent B-cell activation and altered T-cell response. Co-stimulatory and co-inhibitory molecules regulate and exert T-cell differentiation, survival and cytokine production. CD134 and PD-1 T-cells in SLE patients are increased in SLE. The aim of this study was to characterize CD134 and PD-1CD4 T-cells according to their ability to produce IFN-γ, IL-21 and IL-22 in SLE patients.
METHODS
Peripheral blood of 39 SLE patients and 19 healthy controls (HC) was stimulated with phorbol myristate acetate (PMA) calcium ionophore (Ca-Io). The expression of IFN-γ, IL-21 and IL-22 T-cells within the CD134 and PD-1 T-cells was analyzed by flow cytometry. Disease activity was assessed by SLE Disease Activity Index.
RESULTS
Peripheral unstimulated CD134 and PD-1 CD4 T-cells were significantly increased in patients with lupus nephritis. Upon stimulation both, CD134 and PD-1 CD4 T-cells, produced significantly less IFN-γ in SLE patients as compared to HC. The percentages of IL-22 within the CD134CD4 T-cells were also significantly decreased in SLE as compared to HC.
CONCLUSION
CD134 and PD-1CD4 T-cells have mainly a Th1 effector T-cell signature. A lower proportion produces also IL-21 and IL-22. The impaired capacity to produce IFN-γ and IL-22 in SLE patients may contribute to the pathogenesis of the disease.
背景
系统性红斑狼疮（SLE）是一种自身免疫性疾病，其特征是 T 细胞依赖性 B 细胞激活和 T 细胞反应改变。共刺激和共抑制分子调节并发挥 T 细胞分化、存活和细胞因子产生的作用。SLE 患者的 CD134 和 PD-1 T 细胞增加。本研究的目的是根据 SLE 患者产生 IFN-γ、IL-21 和 IL-22 的能力来描述 CD134 和 PD-1CD4 T 细胞。
方法
用佛波醇肉豆蔻酸乙酯（PMA）钙离子载体（Ca-Io）刺激 39 例 SLE 患者和 19 例健康对照（HC）的外周血。通过流式细胞术分析 CD134 和 PD-1 T 细胞内 IFN-γ、IL-21 和 IL-22 T 细胞的表达。用 SLE 疾病活动指数评估疾病活动度。
结果
狼疮肾炎患者外周未刺激的 CD134 和 PD-1 CD4 T 细胞明显增加。与 HC 相比，刺激后 CD134 和 PD-1 CD4 T 细胞产生的 IFN-γ 明显减少。与 HC 相比，SLE 患者的 CD134CD4 T 细胞中 IL-22 的比例也明显降低。
结论
CD134 和 PD-1CD4 T 细胞主要具有 Th1 效应 T 细胞特征。产生 IL-21 和 IL-22 的比例也较低。SLE 患者产生 IFN-γ 和 IL-22 的能力受损可能有助于疾病的发病机制。
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