Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Myeloma Center, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Sci Rep. 2019 Jul 22;9(1):10554. doi: 10.1038/s41598-019-47002-0.
The human kidney embryonic 293 cell line (293 cells) is extensively used in biomedical and pharmaceutical research. These cells exhibit a number of numerical and structural chromosomal anomalies. However, the breakpoints responsible for these structural chromosomal rearrangements have not been comprehensively characterized. In addition, it is not known whether chromosomes with structural rearrangement are more sensitive to external toxic agents, such as ionizing radiation. We used G-banding, spectral karyotyping (SKY), and locus- and region-specific fluorescence in situ hybridization (FISH) probes designed in our lab or obtained from commercial vendor to address this gap. Our G-banding analysis revealed that the chromosome number varies from 66 to 71, with multiple rearrangements and partial additions and deletions. SKY analysis confirmed 3 consistent rearrangements, two simple and one complex in nature. Multicolor FISH analysis identified an array of breakpoints responsible for locus- and region-specific translocations. Finally, SKY analysis revealed that radio-sensitivity of structurally rearranged chromosomes is dependent on radiation dose. These findings will advance our knowledge in 293 cell biology and will enrich the understanding of radiation biology studies.
人胚肾 293 细胞系（293 细胞）广泛应用于生物医学和药物研究。这些细胞表现出许多数量和结构染色体异常。然而，导致这些结构染色体重排的断裂点尚未得到全面描述。此外，尚不清楚是否具有结构重排的染色体对外界有毒剂（如电离辐射）更敏感。我们使用 G 带、光谱核型分析（SKY）和我们实验室设计或从商业供应商获得的基因座和区域特异性荧光原位杂交（FISH）探针来解决这一差距。我们的 G 带分析显示染色体数量从 66 到 71 不等，存在多种重排和部分缺失和添加。SKY 分析证实了 3 个一致的重排，其中 2 个是简单的，1 个是复杂的。多色 FISH 分析确定了一系列导致基因座和区域特异性易位的断裂点。最后，SKY 分析显示，结构重排染色体的放射敏感性取决于辐射剂量。这些发现将推进我们对 293 细胞生物学的认识，并丰富对放射生物学研究的理解。
