Owczarczyk-Saczonek Agnieszka, Czerwińska Joanna, Orylska Małgorzata, Placek Waldemar
Department of Dermatology, Sexually Transmitted Diseases and Clinical Immunology, University of Warmia and Mazury, Olsztyn, Poland.
Postepy Dermatol Alergol. 2019 Jun;36(3):319-328. doi: 10.5114/ada.2019.85641. Epub 2019 Jun 19.
Psoriasis is an autoimmune disease with an excessively aberration of the Th17/Treg balance and deficiency of anti-inflammatory cytokines.
Evaluation of Treg markers expression in the lesional and perilesional psoriatic skin and serum anti-inflammatory cytokines in male psoriatic patients compared to healthy men.
Treg markers (FoxP3+, CD4, CTLA-4, CD25/IL-2R, CD39/ENTPD1, IL-7R/CD127, CD3) and tissue expression of protective cytokines (IL-10, IL-35, TGF-β) in the lesional and perilesional psoriatic skin from 33 male patients compared to 6 healthy skin samples were evaluated by immunohistochemistry. ELISA was used to assess serum IL-10, IL-35 and TGF-β levels.
The serum levels of IL-35, IL-10 and TGF-β1 were higher in psoriatic patients than in controls but without any statistically significant relationship with PASI. The expressions of IL-35, CD4, IL-10, TGF-β1, CD3, FOXP3 and CD25/IL-2R were varied in different experimental groups ( < 0.05). The level of IL-35 was the lowest in psoriatic lesions ( < 0.05) compared to perilesional skin and to controls. CD4, IL-10 and TGF-β1 expressions were higher ( < 0.05) in perilesional skin than in lesions. TGF-β1 expression was decreased in psoriatic lesions compared to controls ( < 0.05). CD25/IL2R expression was increased in healthy skin compared to psoriatic skin ( < 0.05). FOXP3 expression was elevated in psoriatic skin compared to healthy and perilesional one. There was no difference between experimental groups in CTLA-4, IL7R/CD127 and CD39/ENTPD1 expression.
The differences between the levels of protective cytokines and expression of Treg markers might explain the inflammation development in psoriasis.
银屑病是一种自身免疫性疾病，Th17/Treg平衡过度失调且抗炎细胞因子缺乏。
与健康男性相比，评估男性银屑病患者皮损及皮损周边皮肤中Treg标志物的表达情况以及血清抗炎细胞因子水平。
通过免疫组织化学评估33例男性患者的皮损及皮损周边银屑病皮肤中Treg标志物（FoxP3+、CD4、CTLA-4、CD25/IL-2R、CD39/ENTPD1、IL-7R/CD127、CD3）以及保护性细胞因子（IL-10、IL-35、TGF-β）的组织表达情况，并与6份健康皮肤样本作比较。采用酶联免疫吸附测定法评估血清IL-10、IL-35和TGF-β水平。
银屑病患者血清IL-35、IL-10和TGF-β1水平高于对照组，但与银屑病面积和严重程度指数（PASI）无统计学显著相关性。不同实验组中IL-35、CD4、IL-10、TGF-β1、CD3、FOXP3和CD25/IL-2R的表达存在差异（P<0.05）。与皮损周边皮肤及对照组相比，银屑病皮损中IL-35水平最低（P<0.05）。皮损周边皮肤中CD4、IL-10和TGF-β1的表达高于皮损处（P<0.05）。与对照组相比，银屑病皮损中TGF-β1表达降低（P<0.05）。与银屑病皮肤相比，健康皮肤中CD25/IL2R表达增加（P<0.05）。与健康皮肤及皮损周边皮肤相比，银屑病皮肤中FOXP3表达升高。实验组之间CTLA-4, IL7R/CD127和CD39/ENTPD1表达无差异。
保护性细胞因子水平和Treg标志物表达的差异可能解释了银屑病炎症的发展。
