Karamehic Jasenko, Zecevic Lamija, Resic Halima, Jukic Majda, Jukic Tomislav, Ridjic Ognjen, Panjeta Mirsad, Coric Jozo
Department of Immunology, Clinical Center University of Sarajevo, Bosnia and Herzegovina.
Clinical Hemodialysis, Clinical Center of University Sarajevo, Bosnia and Herzegovina.
Med Arch. 2014 Aug;68(4):236-8. doi: 10.5455/medarh.2014.68.236-238. Epub 2014 Jul 31.
Regulatory T cells (Treg) play a central role in the immunopathogenesis of psoriasis. Immunoregulatory T cells (Tregs) are involved in important homeostatic mechanism for maintaining tolerance and preventing autoimmunity, and autoimmune diseases. The aim of this study was to examine the role of Tregs cells in the pathogenesis of psoriasis, and determine the range value for Treg cells (CD4+ CD25+) in the peripheral blood of patients with psoriasis compared to the severity of disease.
The study included 51 patients diagnosed with psoriasis and 25 healthy individuals. Phenotype profile of peripheral blood lymphocytes was determined by flow cytometry, and assessment of severity of disease was determined on the basis of PASI score (e.g. Psoriasis Area and Severity Index).
Proportion of CD4+CD25+T cells in the control group was significantly higher than in the patients with psoriasis [6,4% ±(5,4-7,6) vs. 4,1% (3,1 -5,8)-Mann-Whitney U test, p <0.001]. In the present study we did not find a statistically significant correlation between the levels of CD4+CD25+cells, in patients with psoriasis, compared to the severity of disease-PASI. (i.e. Pearson correlation, r = 0.197, p = 0.194).
The stratification of patients, according to the severity of the clinical course was not possible on the basis of Treg cells' level. ROC curve analysis of the optimal cutoff (PASI=10) and the CD4+CD25+, which distinguishes between patients and healthy individuals was 5% of CD4+CD25+ of the total number of CD4+ lymphocytes with specificity of 69% and sensitivity of 84%.
调节性T细胞（Treg）在银屑病的免疫发病机制中起核心作用。免疫调节性T细胞（Tregs）参与维持耐受性、预防自身免疫和自身免疫性疾病的重要稳态机制。本研究的目的是探讨Tregs细胞在银屑病发病机制中的作用，并确定银屑病患者外周血中Treg细胞（CD4 + CD25 +）的范围值与疾病严重程度的关系。
该研究纳入了51例诊断为银屑病的患者和25名健康个体。通过流式细胞术测定外周血淋巴细胞的表型特征，并根据PASI评分（如银屑病面积和严重程度指数）评估疾病严重程度。
对照组中CD4 + CD25 + T细胞的比例显著高于银屑病患者[6.4%±（5.4 - 7.6）对4.1%（3.1 - 5.8）-曼-惠特尼U检验，p <0.001]。在本研究中，与疾病严重程度-PASI相比，我们未发现银屑病患者中CD4 + CD25 +细胞水平之间存在统计学显著相关性。（即皮尔逊相关性，r = 0.197，p = 0.194）。
根据Treg细胞水平无法对患者按临床病程严重程度进行分层。区分患者和健康个体的最佳截断值（PASI = 10）和CD4 + CD25 +的ROC曲线分析显示，CD4 + CD25 +占CD4 +淋巴细胞总数的5%，特异性为69%，敏感性为84%。
