Chauhan Deepika, Srivastava Pulkit Anupam, Ritzl Barbara, Yennamalli Ragothaman M, Cava Felipe, Priyadarshini Richa
Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Greater Noida, India.
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, India.
Front Microbiol. 2019 Jul 3;10:1449. doi: 10.3389/fmicb.2019.01449. eCollection 2019.
exhibits growth medium-dependent morphological variation in cell shape, but there is no evidence whether this phenomenon is observed in other members of the Deinococcaceae family. In this study, we isolated a red-pigmented, aerobic, strain DR1 from Dadri wetland, India. This strain exhibited cell-morphology transition from rod-shaped cells to multi-cell chains in a growth-medium-dependent fashion. In response to addition of 1% casamino acids in the minimal growth medium, rod-shaped cells formed multi-cell chains. Addition of all 20 amino acids to the minimal medium was able to recapitulate the phenotype. Specifically, a combination of L-methionine, L-lysine, L-aspartate, and L-threonine caused morphological alterations. The transition from rod shape to multi-cell chains is due to delay in daughter cell separation after cell division. Minimal medium supplemented with L-ornithine alone was able to cause cell morphology changes. Furthermore, a comparative UPLC analysis of PG fragments isolated from cells propagated in different growth media revealed alterations in the PG composition. An increase in the overall cross-linkage of PG was observed in muropeptides from nutrient-rich TSB and NB media versus PYE medium. Overall our study highlights that environmental conditions influence PG composition and cell morphology in .
该菌株在细胞形态上表现出生长培养基依赖性的变化，但尚无证据表明在嗜热放线菌科的其他成员中是否也观察到这种现象。在本研究中，我们从印度达德里湿地分离出一株红色色素、需氧的菌株DR1。该菌株以生长培养基依赖性方式表现出从杆状细胞到多细胞链的细胞形态转变。在基本生长培养基中添加1%的酪蛋白氨基酸后，杆状细胞形成了多细胞链。向基本培养基中添加所有20种氨基酸能够重现该表型。具体而言，L-甲硫氨酸、L-赖氨酸、L-天冬氨酸和L-苏氨酸的组合导致了形态改变。从杆状到多细胞链的转变是由于细胞分裂后子细胞分离延迟。仅添加L-鸟氨酸的基本培养基就能引起细胞形态变化。此外，对在不同生长培养基中繁殖的细胞分离出的肽聚糖片段进行的比较超高效液相色谱分析显示，肽聚糖组成发生了改变。与PYE培养基相比，在富含营养的TSB和NB培养基中的胞壁肽中观察到肽聚糖的总体交联增加。总体而言，我们的研究强调环境条件会影响肽聚糖组成和该菌株的细胞形态。
