Suresh K, Reddy G S N, Sengupta S, Shivaji S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Geological Survey of India, GSI Complex, DK-6, Sector-II, Bidhannagar, Kolkata 700 091, India.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):457-461. doi: 10.1099/ijs.0.02758-0.
An arsenic- and radiation-resistant bacterium, strain Wt/1a(T), was isolated from water from an arsenic-contaminated aquifer located in the Chakdah district of West Bengal, India. The bacterium stains Gram-negative and is rod-shaped, non-motile, non-sporulating and red-pigmented. Cell-wall peptidoglycan contains ornithine as the diamino acid, MK-8 is the major menaquinone, C(15 : 1) and C(16 : 1) are the major fatty acids and the DNA G+C content of the organism is 65.8 mol%. Based on these phenotypic and chemotaxonomic characteristics, strain Wt/1a(T) was identified as a member of the genus Deinococcus. Strain Wt/1a(T) exhibited maximum 16S rRNA gene sequence similarity (95 %) with Deinococcus grandis; however, strain Wt/1a(T) exhibited only 14 % similarity to D. grandis IAM 13005(T) at the DNA-DNA level. Furthermore, strain Wt/1a(T) (compared to D. grandis IAM 13005(T)) is more resistant to arsenate and arsenite, is positive for arginine dihydrolase, utilizes a number of carbon sources and exhibits quantitative differences in fatty acid composition and qualitative differences in lipid composition. Strain Wt/1a(T) is identified as a novel species of the genus Deinococcus, for which the name Deinococcus indicus sp. nov. is proposed. The type strain of Deinococcus indicus is Wt/1a(T) (=MTCC 4913(T)=DSM 15307(T)).
从印度西孟加拉邦查克达地区受砷污染的含水层水中分离出一株抗砷和抗辐射细菌，菌株Wt/1a(T)。该细菌革兰氏染色阴性，呈杆状，无运动性，不产芽孢，有红色色素。细胞壁肽聚糖含有鸟氨酸作为二氨基酸，MK-8是主要的甲基萘醌，C(15 : 1)和C(16 : 1)是主要脂肪酸，该生物体的DNA G+C含量为65.8 mol%。基于这些表型和化学分类特征，菌株Wt/1a(T)被鉴定为嗜皮菌属的一个成员。菌株Wt/1a(T)与巨大嗜皮菌的16S rRNA基因序列相似性最高（95%）；然而，在DNA-DNA水平上，菌株Wt/1a(T)与巨大嗜皮菌IAM 13005(T)的相似性仅为14%。此外，菌株Wt/1a(T)（与巨大嗜皮菌IAM 13005(T)相比）对砷酸盐和亚砷酸盐更具抗性，精氨酸双水解酶呈阳性，利用多种碳源，并且在脂肪酸组成上存在数量差异，在脂质组成上存在质量差异。菌株Wt/1a(T)被鉴定为嗜皮菌属的一个新物种，为此提出名称嗜皮菌印度种（Deinococcus indicus sp. nov.）。嗜皮菌印度种的模式菌株是Wt/1a(T)（=MTCC 4913(T)=DSM 15307(T)）。
