Nejad Jalil Ghassemi, Ataallahi Mohammad, Park Kyu-Hyun
College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea.
Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul 05029, Korea.
J Anim Sci Technol. 2019 Jan;61(1):41-46. doi: 10.5187/jast.2019.61.1.41. Epub 2019 Jan 31.
Different methodologies in hair cortisol extraction may alter the final output. Thus finding the standard methodology according to a laboratory facilities is pivotal. This study was carried out to validate the feasibility of two methods of grinding hair for cortisol extraction in Korean native (Hanwoo) cattle. Hair from nine cattle including mature cows, heifers, and calves were assigned to one of the following methods for grinding hair; 1) using bead beater (BB) and 2) using surgical scissors (SS). Hair samples (> 1 g) were harvested from forehead of each individual twice (first and second measurement) to validate the results. To improve the accuracy of the obtained data, each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis. Overall comparison of hair cortisol concentration (HCC) showed that the data within the range (out of the range) of standards provided by the EIA kit were 88.9% (11.1%) and 66.7% (33.3%) for BB compared with SS, respectively. In the first measurement, application of BB was tended to show higher ( = 0.056) amount of HCC compared with SS. In the second measurement application of BB showed higher ( = 0.0028) amount of HCC compared with SS. Among the cattle, calves showed higher HCC using BB compared with SS ( < 0.05). Application of BB in hair grinding methodology for Hanwoo cattle may improve cortisol extraction in comparison to application of SS method, with more consistency. Thus, it would be the preferable method to use.
毛发皮质醇提取的不同方法可能会改变最终结果。因此，根据实验室设施找到标准方法至关重要。本研究旨在验证韩国本土（韩牛）牛毛发皮质醇提取的两种毛发研磨方法的可行性。将包括成年母牛、小母牛和小牛在内的九头牛的毛发分配到以下毛发研磨方法之一；1） 使用珠磨仪（BB）和2） 使用手术剪刀（SS）。从每头牛的额头采集毛发样本（>1g）两次（第一次和第二次测量）以验证结果。为了提高所获数据的准确性，在酶免疫分析（EIA）过程中，每个样本被复制到两个孔中。毛发皮质醇浓度（HCC）的总体比较表明，与SS相比，EIA试剂盒提供的标准范围内（范围外）的数据，BB分别为88.9%（11.1%）和66.7%（33.3%）。在第一次测量中，与SS相比，使用BB时HCC含量有更高的趋势（P = 0.056）。在第二次测量中，与SS相比，使用BB时HCC含量更高（P = 0.0028）。在这些牛中，与SS相比，使用BB时小牛的HCC更高（P < 0.05）。与使用SS方法相比，在韩牛毛发研磨方法中使用BB可能会提高皮质醇提取率，且一致性更高。因此，这将是更可取的使用方法。
