Pakbaz Behfar, Jabinin Raheleh, Soltani Narjes, Ayatollahi Hossein, Farzanehfar Mohammad Reza
Department of Gastroenterology and Pathology and Cytogenetic, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
J Adv Pharm Technol Res. 2019 Jul-Sep;10(3):121-125. doi: 10.4103/japtr.JAPTR_381_18.
Due to importance of screening for colorectal cancer the present study was conducted and aimed at investigating the quantitative methylation of vimentin gene in stool, tumor tissue, and healthy tissue of patients with colorectal cancer (CRC) in comparison with healthy individuals. This is a case-control study in which we measures methylation of the vimentin in tumoral tissue, normal tissue and stool specimens were collected from forty-nine CRC patients as case group and stool and normal tissue specimens were collected from thirty healthy individuals as control group. There was no statistically significant difference in methylation of vimentin in normal tissue ( > 0.05) between the two groups. Moreover, the status of methylated or unmethylated vimentin gene in tumor and stool tissues in the case group was not significantly correlated with their mean age and sex ( > 0.05). This study showed that the specificity and sensitivity of vimentin methylation in stool of CRC patients are 100% and 60%, respectively. Furthermore, the methylation of vimentin in stool of CRC patients has a high-positive predictive value (100%). The results of this study suggested that methylation of the vimentin gene in the stool can be used as a specific marker for the detection and screening of CRC.
由于结直肠癌筛查的重要性，开展了本研究，旨在调查结直肠癌（CRC）患者粪便、肿瘤组织和健康组织中波形蛋白基因的定量甲基化情况，并与健康个体进行比较。这是一项病例对照研究，我们测定了49例CRC患者作为病例组的肿瘤组织、正常组织和粪便标本中波形蛋白的甲基化情况，以及30例健康个体作为对照组的粪便和正常组织标本中波形蛋白的甲基化情况。两组之间正常组织中波形蛋白的甲基化无统计学显著差异（>0.05）。此外，病例组肿瘤和粪便组织中波形蛋白基因的甲基化或未甲基化状态与它们的平均年龄和性别无显著相关性（>0.05）。本研究表明，CRC患者粪便中波形蛋白甲基化的特异性和敏感性分别为100%和60%。此外，CRC患者粪便中波形蛋白的甲基化具有较高的阳性预测值（100%）。本研究结果表明，粪便中波形蛋白基因的甲基化可作为CRC检测和筛查的特异性标志物。
