Guo Wei, Huang Qiulan, Liu Hao, Hou Shaoli, Niu Suhao, Jiang Yi, Bao Xiaoming, Shen Yu, Fang Xu
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science and Technology, Ministry of Education, Tianjin, China.
Front Bioeng Biotechnol. 2019 Jul 3;7:152. doi: 10.3389/fbioe.2019.00152. eCollection 2019.
Tyrosol is extensively used in the pharmaceutical industry as an important natural product from plants. In this study, an exogenous pathway involved in catalyzing tyrosine to tyrosol was introduced into . Furthermore, The pyruvate decarboxylase gene was deleted to redirect the flux distribution at the pyruvate node, and a bifunctional NAD-dependent fused chorismate mutase/prephenate dehydrogenase from (TyrA) and its' tyrosine inhibition resistant mutant (TyrA) were heterologously expression in to tuning up the chorismate metabolism effectively directed the metabolic flux toward tyrosol production. Finally, the tyrosol yield of the engineered strain GFT-4 was improved to 126.74 ± 6.70 mg/g DCW at 48 h, increased 440 times compared with that of the control strain GFT-0 (0.28 ± 0.01 mg/g DCW). The new synergetic engineering strategy developed in this study can be further applied to increase the production of high value-added aromatic compounds derived from aromatic amino acid or shikimate in .
酪醇作为一种重要的植物天然产物，在制药工业中被广泛应用。在本研究中，一条催化酪氨酸生成酪醇的外源途径被引入到……此外，丙酮酸脱羧酶基因被删除以重新分配丙酮酸节点处的通量分布，来自……的双功能NAD依赖型融合分支酸变位酶/预苯酸脱氢酶（TyrA）及其酪氨酸抑制抗性突变体（TyrA）在……中进行异源表达，以有效调节分支酸代谢，将代谢通量导向酪醇的生产。最终，工程菌株GFT - 4在48小时时酪醇产量提高到126.74±6.70 mg/g DCW，与对照菌株GFT - 0（0.28±0.01 mg/g DCW）相比增加了440倍。本研究中开发的新的协同工程策略可进一步应用于提高……中源自芳香族氨基酸或莽草酸途径的高附加值芳香族化合物的产量。
