Esfandi Farbod, Fallah Hamid, Arsang-Jang Shahram, Taheri Mohammad, Ghafouri-Fard Soudeh
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
GenIran Lab, Tashkhis Gene Pajohesh, Tehran, Iran.
Rep Biochem Mol Biol. 2019 Apr;8(1):36-41.
Long non-coding RNAs (lncRNAs) have been considered to be prospective biomarkers for diagnosing lung cancer due to the fundamental roles they hold in the regulating several cancer-related pathways.
Using the quantitative real-time polymerase chain reaction method, we evaluated the expression of and lncRNAs in 32 lung cancer tissue samples and their corresponding adjacent non-cancerous tissues (ANCTs) from lung cancer patients admitted to the Labbafi-Nejad Hospital from 2015 to 2016.
No significant differences were found in the expression of lncRNAs within the tumoral and non-tumoral tissue samples. Bayesian Multilevel analysis showed no association between the expression of lncRNAs and the patient's tumor node metastasis (TNM) stage following adjustments for age. Spearman correlation analysis revealed an inverse correlation between the expression of in tumoral tissues and age. Additionally, the difference in expression among the tumoral and non-tumoral tissues was inversely correlated with patients' age. Significant pairwise correlations were found between the expression of lncRNAs in both the tumoral and non-tumoral tissues.
Despite the findings supporting a role for the lncRNAs, and in the pathogenesis of lung cancer, our data suggests no relationship for expression of these lncRNAs in lung cancer, questioning their potential as lung cancer biomarkers.
长链非编码RNA（lncRNAs）因其在调控多种癌症相关通路中发挥的重要作用，被认为是诊断肺癌的潜在生物标志物。
采用定量实时聚合酶链反应方法，我们评估了2015年至2016年入住拉巴菲 - 内贾德医院的肺癌患者的32份肺癌组织样本及其相应的癌旁非癌组织（ANCTs）中lncRNAs的表达情况。
肿瘤组织样本和非肿瘤组织样本中lncRNAs的表达未发现显著差异。贝叶斯多水平分析显示，在对年龄进行调整后，lncRNAs的表达与患者的肿瘤淋巴结转移（TNM）分期之间没有关联。Spearman相关性分析显示，肿瘤组织中lncRNAs的表达与年龄呈负相关。此外，肿瘤组织和非肿瘤组织中lncRNAs表达的差异与患者年龄呈负相关。在肿瘤组织和非肿瘤组织中，lncRNAs的表达之间存在显著的成对相关性。
尽管研究结果支持lncRNAs在肺癌发病机制中发挥作用，但我们的数据表明这些lncRNAs在肺癌中的表达与肺癌无关，这对它们作为肺癌生物标志物的潜力提出了质疑。
