Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Am J Hum Biol. 2019 Nov;31(6):e23298. doi: 10.1002/ajhb.23298. Epub 2019 Jul 23.
A handheld biosensor for measuring salivary α-amylase (sAA) was developed for convenient on-site measurement. Previous studies reported some discrepancies in sAA levels measured with a biosensor and a standard assay. This study aimed to compare sAA levels measured with three different methods and the factors affecting its levels.
Thirty-eight participants collected saliva two times for three measurements. First, the collector strip was placed under the tongue for 2 minutes, then the strip was used to measure sAA level on-site immediately (intraoral biosensor; method 1). Then, a participant pooled the saliva for 4 minutes and collected the saliva into the tube which was aliquoted to measure in a laboratory with a handheld biosensor (extraoral biosensor; method 2) and with a standard enzyme kinetic assay (EKA; method 3). Additional experiments were carried out to compare the levels of sAA measured with differences in pooling time and positioning of the collector strip.
A high correlation of sAA levels between an extraoral and an EKA measurement (r = 0.989) was observed, while sAA levels measured with an intraoral method showed a significant but weaker correlation with either an EKA (r = 0.475) or an extraoral method (r = 0.436). Saliva pooling time and positioning of the collector strip significantly affected sAA levels.
A handheld biosensor is valid to measure sAA levels extraorally. For an intraoral measurement, pooling time and positioning of the collector strip need to be taken into account.
开发了一种用于方便现场测量的手持式唾液α-淀粉酶（sAA）生物传感器。先前的研究报告了生物传感器和标准测定法测量的 sAA 水平存在一些差异。本研究旨在比较三种不同方法测量的 sAA 水平及其影响因素。
38 名参与者采集唾液进行三次测量，每次采集两次。首先，采集器条放置在舌下 2 分钟，然后立即使用条在现场测量 sAA 水平（口腔内生物传感器；方法 1）。然后，参与者将唾液混合 4 分钟，将唾液收集到管中，然后将其等分，在实验室使用手持式生物传感器（口腔外生物传感器；方法 2）和标准酶动力学测定法（EKA；方法 3）进行测量。进行了额外的实验，以比较不同的混合时间和采集器条放置位置测量的 sAA 水平。
观察到口腔外和 EKA 测量的 sAA 水平高度相关（r = 0.989），而口腔内方法测量的 sAA 水平与 EKA（r = 0.475）或口腔外方法（r = 0.436）的相关性显著但较弱。唾液混合时间和采集器条的放置位置显著影响 sAA 水平。
手持式生物传感器可有效测量口腔外的 sAA 水平。对于口腔内测量，需要考虑混合时间和采集器条的放置位置。
