Peng Henry T, Savage Erin, Vartanian Oshin, Smith Shane, Rhind Shawn G, Tenn Catherine, Bjamason Stephen
Defence Research and Development Canada, Toronto Research Centre, Toronto, Ontario, Canada.
Canadian Forces Health Services, Ottawa, Ontario, Canada.
J Clin Lab Anal. 2016 May;30(3):223-30. doi: 10.1002/jcla.21840. Epub 2015 Jan 19.
A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two different modalities in operating room and field settings.
Salivary amylase activity was measured by two biosensor methods: directly sampling saliva with a test strip placed under the tongue or pipetting a fixed volume of precollected saliva onto the test strip, followed by analyzing the sample on the strip using a biosensor. The two methods were compared for their accuracy and sensitivity to detect the stress response using an enzyme assay method as a standard.
The measurements from the under-the-tongue method were not as consistent with those from the standard assay method as the values obtained from the pipetting method. The under-the-tongue method did not detect any significant increase in the amylase activity due to stress in the operating room (P > 0.1), in contrast to the significant increases observed using the pipetting method and assay method with a significance level less than 0.05 and 0.1, respectively. Furthermore, the under-the-tongue method showed no increased amylase activity in the field testing, while both the pipetting method and assay method showed increased amylase activity in the same group (P < 0.1).
The accuracy and consistency of the biosensors need to be improved when used to directly measure salivary amylase activity under the tongue for stress assessment in military medical training.
对于现场心理生理应激研究和军事行动而言，一种用于实时测量生物标志物的便捷生物传感器是很有必要的。我们评估了一种手持设备，该设备可在手术室和野外环境中通过两种不同方式，测量接受战斗伤员护理培训的医疗技术人员唾液淀粉酶作为应激标志物。
通过两种生物传感器方法测量唾液淀粉酶活性：一种是将测试条置于舌下直接采集唾液，另一种是将固定体积的预先采集的唾液移液到测试条上，然后使用生物传感器在测试条上分析样本。以酶测定法作为标准，比较这两种方法检测应激反应的准确性和灵敏度。
舌下测量法得到的结果与标准测定法的结果相比，不如移液法得到的值一致。舌下测量法未检测到由于手术室中的应激导致淀粉酶活性有任何显著增加（P>0.1），相比之下，移液法和测定法分别在显著性水平小于0.05和0.1时观察到了显著增加。此外，在现场测试中，舌下测量法未显示淀粉酶活性增加，而移液法和测定法在同一组中均显示淀粉酶活性增加（P<0.1）。
在军事医学训练中用于通过舌下直接测量唾液淀粉酶活性进行应激评估时，生物传感器的准确性和一致性需要提高。
