Centro de Investigaciones de Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, México.
Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, México.
Microb Drug Resist. 2019 Sep;25(7):1023-1031. doi: 10.1089/mdr.2019.0034. Epub 2019 Jul 26.
complex isolates have been frequently associated with hospital and community infections, with being the most common. Other spp. not belonging to this complex also cause infections in hospital settings, and the incidence has increased over the past few years. Some species of the genus possess a great diversity of antibiotic resistance mechanisms, such as efflux pumps, porins, and resistance genes that can be acquired and disseminated by mobilizable genetic elements. By means of whole-genome sequencing, we describe in the clinical strain AN54 different mechanisms of resistance that involve , , , , and a resistance-nodulation-cell division-type efflux pump. This strain carries six plasmids, of which the plasmid pAhaeAN54e contains in a Tn125-like transposon that is truncated at the 3' end. This strain also has an insertion sequence IS and seven genes encoding hypothetical proteins. The pAhaeAN54e plasmid is nontypable and different from other plasmids carrying that have been reported in Mexico and other countries. The presence of these kinds of plasmids in an opportunistic pathogen such as highlights the role that these plasmids play in the dissemination of antibiotic resistance genes, especially against carbapenems, in Mexican hospitals.
复杂的分离株经常与医院和社区感染有关，其中 是最常见的。其他不属于该复合体的 spp. 也会导致医院感染，近年来发病率有所增加。一些 属的物种具有多种抗生素耐药机制，如外排泵、孔蛋白和可通过可移动遗传元件获得和传播的耐药基因。通过全基因组测序，我们在临床 株 AN54 中描述了涉及 、 、 、 、和一种耐药-结节-分裂型外排泵的不同耐药机制。该菌株携带六个质粒，其中质粒 pAhaeAN54e 包含在 Tn125 样转座子中，该转座子在 3' 末端截断。该菌株还具有插入序列 IS 和七个编码假定蛋白的基因。pAhaeAN54e 质粒不可分型，与在墨西哥和其他国家报告的携带 的其他质粒不同。这些质粒在机会性病原体如 中的存在突出了这些质粒在传播抗生素耐药基因方面的作用，特别是对墨西哥医院中碳青霉烯类抗生素的耐药基因。
