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p75，一种与黑腹果蝇多线染色体转录活性位点相关的富含核骨架蛋白组分中的多肽成分。

p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes.

作者信息

Benton B M, Berrios S, Fisher P A

机构信息

Graduate Program in Cellular and Developmental Biology, State University of New York, Stony Brook 11794-8651.

出版信息

Mol Cell Biol. 1988 May;8(5):1877-86. doi: 10.1128/mcb.8.5.1877-1886.1988.

DOI:10.1128/mcb.8.5.1877-1886.1988
PMID:3133549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363365/
Abstract

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

摘要

已鉴定出一种75千道尔顿的多肽，它与从黑腹果蝇胚胎制备的富含核骨架蛋白的组分共纯化。间接免疫荧光实验结果表明，这种蛋白质（此处命名为p75）主要与幼虫唾液腺多线染色体的胀泡区域相关。在非多线化的施耐德2组织培养细胞中，p75在间期似乎定位于整个核内。在有丝分裂细胞中，p75呈弥散性重新分布。本文讨论了核骨架成分在转录调控中的可能作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/40dcd60f2f42/molcellb00065-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/bab058e33f98/molcellb00065-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/1de99c66e315/molcellb00065-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/790cb4ae9202/molcellb00065-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/681c65dac85b/molcellb00065-0031-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/1c72d30c7536/molcellb00065-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/238f9cdbc882/molcellb00065-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/40dcd60f2f42/molcellb00065-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/bab058e33f98/molcellb00065-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/1de99c66e315/molcellb00065-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/790cb4ae9202/molcellb00065-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/681c65dac85b/molcellb00065-0031-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/1c72d30c7536/molcellb00065-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/238f9cdbc882/molcellb00065-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a10/363365/40dcd60f2f42/molcellb00065-0034-a.jpg

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Isolation and distribution of a Drosophila protein preferentially associated with active regions of the genome.一种与基因组活性区域优先相关的果蝇蛋白质的分离与分布
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本文引用的文献

1
Identification of a major polypeptide of the nuclear pore complex.核孔复合体一种主要多肽的鉴定。
J Cell Biol. 1982 Dec;95(3):826-37. doi: 10.1083/jcb.95.3.826.
2
RNA polymerase II transcribes all of the heat shock induced genes of Drosophila melanogaster.RNA聚合酶II转录黑腹果蝇所有热休克诱导基因。
Chromosoma. 1982;85(1):93-108. doi: 10.1007/BF00344596.
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A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.一种在蛋白质免疫印迹法中检测碱性磷酸酶偶联抗抗体的快速、灵敏方法。
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Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.果蝇胚胎中一种主要核被膜蛋白的两种抗原相关形式的鉴定、发育调控及对热休克的反应：利用固定在硝酸纤维素印迹上的多肽改进抗体亲和纯化方法的应用
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Small nuclear ribonucleoproteins of Drosophila: identification of U1 RNA-associated proteins and their behavior during heat shock.果蝇的小核核糖核蛋白：U1 RNA相关蛋白的鉴定及其在热休克期间的行为
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Isolation and characterization of a proteinaceous subnuclear fraction composed of nuclear matrix, peripheral lamina, and nuclear pore complexes from embryos of Drosophila melanogaster.从黑腹果蝇胚胎中分离并鉴定一种由核基质、核周层和核孔复合体组成的蛋白质性亚核组分。
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Immunological studies of RNA polymerase II using antibodies to subunits of Drosophila and wheat germ enzyme.利用针对果蝇和小麦胚芽酶亚基的抗体对RNA聚合酶II进行的免疫学研究。
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Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.源自具有狼疮样自身免疫的小鼠品系的抗RNP单克隆抗体。
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A 174-kilodalton ATPase/dATPase polypeptide and a glycoprotein of apparently identical molecular weight are common but distinct components of higher eukaryotic nuclear structural protein subfractions.一种174千道尔顿的ATP酶/dATP酶多肽和一种分子量明显相同的糖蛋白是高等真核细胞核结构蛋白亚组分中常见但不同的成分。
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