Cohen D R, Curran T
Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Mol Cell Biol. 1988 May;8(5):2063-9. doi: 10.1128/mcb.8.5.2063-2069.1988.
A set of proteins antigenically related to the c-fos protein (Fos) are induced by serum in fibroblasts. To isolate cDNA clones of genes encoding such proteins, a lambda gt11 expression cDNA library constructed from serum-stimulated rat fibroblasts was screened with antibodies raised against a hydrophilic region (amino acids 127 to 152) of Fos. One of the positive clones identified, termed fra-1 (Fos-related antigen) was characterized. It encoded a protein that shared several regions of extensive amino acid homology with Fos (including the region that showed similarity to both the yeast GCN4 regulatory protein and the protein encoded by the jun oncogene), although its nucleotide sequence was considerably diverged from that of the c-fos gene. Only a subset of the agents and conditions that activated c-fos also induced fra-1. Induction of fra-1 expression following serum stimulation was delayed compared with that of c-fos. However, like c-fos, fra-1 was induced rapidly by serum in the presence of protein synthesis inhibitors. Thus, a family of Fos-related, inducible genes are involved in the cellular immediate-early transcriptional response to extracellular stimuli.
血清可在成纤维细胞中诱导出一组与c-fos蛋白（Fos）抗原相关的蛋白质。为了分离编码此类蛋白质的基因的cDNA克隆，用针对Fos亲水区域（氨基酸127至152）产生的抗体筛选了由血清刺激的大鼠成纤维细胞构建的λgt11表达cDNA文库。鉴定出的一个阳性克隆，称为fra-1（Fos相关抗原），对其进行了表征。它编码的一种蛋白质与Fos有几个广泛的氨基酸同源区域（包括与酵母GCN4调节蛋白和jun癌基因编码的蛋白质都相似的区域），尽管其核苷酸序列与c-fos基因有很大差异。激活c-fos的因子和条件中只有一部分也能诱导fra-1。与c-fos相比，血清刺激后fra-1表达的诱导延迟。然而，与c-fos一样，在存在蛋白质合成抑制剂的情况下，血清能迅速诱导fra-1。因此，一组Fos相关的可诱导基因参与了细胞对细胞外刺激物的即时早期转录反应。
