Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MOA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640, China.
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (MOA), Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640, China.
Int J Biol Macromol. 2019 Oct 1;138:511-518. doi: 10.1016/j.ijbiomac.2019.07.117. Epub 2019 Jul 20.
Citrus grandis 'Tomentosa' (CGT) which is a region-famous medicinal and edible plant contains plentiful bioactive polysaccharides, however, its chemical structures and specific bioactivities still need to be further explored. In the present study, an acidic polysaccharide (CGTP-AP) was extracted and purified from the pulps of CGT. The structure elucidation and anti-cancer activity of CGTP-AP were investigated. Structure characterization indicated that CGTP-AP was a homogeneous heteropolysaccharide composed of arabinose, galactose and galacturonic acid in a molar ratio of 2.45:1:2.77, with an average molecular weight of 2721.68 kDa. Partial acid hydrolysis, methylation and NMR spectrometry revealed that the backbone of CGTP-AP mainly composed of (1 → 4)-α-D-galacturonan, while the branch principally consisted of (1 → 5)-α-L-Araf. In addition, CGTP-AP exhibited effective anti-proliferation against colon cancer cells LOVO and SW620 cells in dose- and time-dependent manners, with IC values of 5.55, 4.35 and 3.52 mg mL after 24, 48 and 72 h, and 5.33, 3.63 and 2.97 mg mL after 24, 48 and 72 h, respectively. This study indicated that CGTP-AP might be utilized as a promising food supplement for the patients of colon disorders.
柚（Citrus grandis 'Tomentosa'）是一种具有地域特色的药食两用植物，富含丰富的生物活性多糖，但它的化学结构和特定的生物活性仍需进一步研究。本研究从柚果肉中提取并纯化了一种酸性多糖（CGTP-AP）。对 CGTP-AP 的结构进行了鉴定，并研究了其抗癌活性。结构分析表明，CGTP-AP 是一种均一的杂多糖，由阿拉伯糖、半乳糖和半乳糖醛酸以摩尔比 2.45:1:2.77 组成，平均分子量为 2721.68 kDa。部分酸水解、甲基化和 NMR 光谱分析表明，CGTP-AP 的主链主要由（1→4）-α-D-半乳糖醛酸组成，而支链主要由（1→5）-α-L-阿拉伯呋喃糖组成。此外，CGTP-AP 对结肠癌 LOVO 和 SW620 细胞具有有效的增殖抑制作用，呈剂量和时间依赖性，24、48 和 72 h 时的 IC50 值分别为 5.55、4.35 和 3.52 mg/mL，24、48 和 72 h 时的 IC50 值分别为 5.33、3.63 和 2.97 mg/mL。本研究表明，CGTP-AP 可能作为一种有前景的食品补充剂，用于治疗结肠疾病患者。
