Opleta K, Butzner J D, Shaffer E A, Gall D G
Health Science Centre, University of Calgary, Alberta, Canada.
Pediatr Res. 1988 May;23(5):505-8. doi: 10.1203/00006450-198805000-00014.
The effects of protein-calorie malnutrition on bile salt metabolism and liver function were studied. Malnutrition was induced in rabbits by combining litters at 7 days of age (13-16 animals) and results compared to control litters (six-eight animals). At age 29-30 days biliary output from the common bile duct was measured directly for three 1-h periods: under basal conditions, and in response to intravenous infusion of exogenous glycodeoxycholic acid at 0.75 and 1.5 mumol/min/kg, respectively. The bile salt pool size was measured by isotope dilution. Final mean body weight and liver weight were significantly decreased in malnourished animals compared to controls. Liver weight/body weight was also less in the malnourished group. Total liver DNA and protein content, as well as the protein to DNA ratio, were less in the malnourished animals compared to controls. Bile flow and bile salt secretion were reduced in the malnourished group when calculated per kg body weight or per mg liver DNA. Bile salt-dependent flow did not differ significantly, but bile salt-independent flow was significantly less in malnourished animals. Bile salt pool size was decreased in the malnourished group. These findings indicate that malnutrition has a greater impact on liver weight than on total body weight in the preweaning period. In addition, malnutrition reduces bile flow, bile salt secretion, and bile salt pool size which, along with the decrease in bile salt-independent flow, may reflect either an impairment of hepatic uptake function or a delay in postnatal development.
研究了蛋白质-热量营养不良对胆汁盐代谢和肝功能的影响。通过在7日龄时合并同窝仔兔（13 - 16只动物）诱导营养不良，并将结果与对照同窝仔兔（6 - 8只动物）进行比较。在29 - 30日龄时，直接测量胆总管三个1小时期间的胆汁输出量：在基础条件下，以及分别以0.75和1.5 μmol/min/kg的速率静脉输注外源性甘氨脱氧胆酸后的胆汁输出量。通过同位素稀释法测量胆汁盐池大小。与对照组相比，营养不良动物的最终平均体重和肝脏重量显著降低。营养不良组的肝脏重量/体重也较低。与对照组相比，营养不良动物的肝脏总DNA和蛋白质含量以及蛋白质与DNA的比率均较低。按每千克体重或每毫克肝脏DNA计算，营养不良组的胆汁流量和胆汁盐分泌减少。胆汁盐依赖性流量无显著差异，但营养不良动物的胆汁盐非依赖性流量显著减少。营养不良组的胆汁盐池大小减小。这些发现表明，在断奶前时期，营养不良对肝脏重量的影响大于对总体重的影响。此外，营养不良会降低胆汁流量、胆汁盐分泌和胆汁盐池大小，这与胆汁盐非依赖性流量的减少一起，可能反映了肝脏摄取功能的损害或出生后发育的延迟。
