Opleta K, O'Loughlin E V, Shaffer E A, Hayden J, Hollenberg M, Gall D G
Gastrointestinal, Research Group, Faculty of Medicine, Univesity of Calgary, Alberta, Canada.
Am J Physiol. 1987 Nov;253(5 Pt 1):G622-6. doi: 10.1152/ajpgi.1987.253.5.G622.
The effect of epidermal growth factor (EGF) on the postnatal development of the liver was examined. New Zealand White rabbits received 40 micrograms.kg-1.day-1 EGF from days 3 to 17 of age either intraperitoneally or orogastrically, whereas controls received saline. At days 18-20, animals underwent cannulation of the common duct using halothane anesthetic. Biliary output was measured directly for three 1-h periods: under basal conditions and in response to intravenous infusion of exogenous glycodeoxycholic acid at 0.75 and 1.5 mumol.min-1.kg-1, respectively. The bile salt pool size was measured by isotope dilution. Final mean body weight of intraperitoneal and orogastric groups did not differ from controls. Liver we weight, DNA, and protein content were significantly increased in intraperitoneally treated animals without morphological or biochemical evidence of fat deposition. Both intraperitoneal and orogastric EGF significantly increased bile salt secretion in the basal period and as a response to exogenous bile acid infusion. Bile flow was significantly increased in response to 1.5 mumol.min-1.kg-1 infusion of glycodeoxycholic acid. The bile salt pool was increased by both intraperitoneal and orogastric EGF. Administration of EGF resulted in a precocious development of glucokinase (EC 2.7.1.2) activity in the liver. EGF had no effect on serum cortisol, corticosterone, triiodothyronine, thyroxine, or free thyroxine levels. These findings suggest that in the neonatal period EGF can promote hepatic growth and maturation.
研究了表皮生长因子（EGF）对肝脏出生后发育的影响。新西兰白兔在3至17日龄时通过腹腔内或经口胃途径接受40微克·千克⁻¹·天⁻¹的EGF，而对照组接受生理盐水。在18至20日龄时，使用氟烷麻醉对动物进行胆总管插管。直接测量三个1小时期间的胆汁分泌量：在基础条件下以及分别对静脉输注0.75和1.5微摩尔·分钟⁻¹·千克⁻¹的外源性甘氨脱氧胆酸的反应中。通过同位素稀释法测量胆汁盐池大小。腹腔内和经口胃给药组的最终平均体重与对照组无差异。腹腔内给药的动物肝脏重量、DNA和蛋白质含量显著增加，且无脂肪沉积的形态学或生化证据。腹腔内和经口胃给予EGF均显著增加基础期胆汁盐分泌以及对外源性胆汁酸输注的反应。对1.5微摩尔·分钟⁻¹·千克⁻¹的甘氨脱氧胆酸输注反应时胆汁流量显著增加。腹腔内和经口胃给予EGF均使胆汁盐池增加。给予EGF导致肝脏中葡萄糖激酶（EC 2.7.1.2）活性早熟。EGF对血清皮质醇、皮质酮、三碘甲状腺原氨酸、甲状腺素或游离甲状腺素水平无影响。这些发现表明，在新生儿期EGF可促进肝脏生长和成熟。
