分析产自沙特阿拉伯的牛至精油的化学成分，并评估其细胞毒性、抗菌和抗氧化活性。
Analysis of Chemical Composition and Assessment of Cytotoxic, Antimicrobial, and Antioxidant Activities of the Essential Oil of Growing in Saudi Arabia.
机构信息
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Medicinal Aromatic, and Poisonous Plants Research Center, Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
出版信息
Molecules. 2019 Jul 22;24(14):2647. doi: 10.3390/molecules24142647.
The essential oil of (Konig ex Roxb.) Benth. (Synonym: , Lamiaceae) collected from Saudi Arabia was studied utilizing GC and GC/MS. Forty four constituents were identified, representing 96.8% of the total oil. The essential oil (MDEO) was characterized by a high content of oxygenated monoterpenes (76.2%). Camphor (54.3%) was the major compound in MDEO followed by 1,8-cineole (12.2%) and camphene (10.4%). Moreover, MDEO was assessed for its cytotoxic, antimicrobial, and antioxidant activities. MDEO demonstrated an interesting cytotoxic activity against all cancer cell lines with IC values of 83.6 to 91.2 μg/mL, especially against MCF-7 cancer cells. Using labeling with annexin VFITC and/or propidium iodide (PI) dyes and flow cytometer analysis, the apoptosis induction was quantitatively confirmed for MCF-7 cells. The MDEO exhibited a considerable antimicrobial activity against all bacterial and fungal strains with minimum inhibitory concentration (MIC)-values of 0.07 to 1.25 mg/mL. The most sensitive microbial strain was (MIC: 0.07 mg/mL). Minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) values were determined one time higher than that of MIC's. Additionally, the MDEO revealed a strong activity for reducing β-carotene bleaching with a total antioxidant value of 72.6% and significant DPPH free radical scavenging activity (78.4%) at the concentration 1000 μg/mL.
从沙特阿拉伯采集的 （Konig ex Roxb.）Benth.（同义词：，唇形科）精油利用 GC 和 GC/MS 进行了研究。鉴定出 44 种成分，占总油的 96.8%。该精油（MDEO）的特点是含氧单萜含量高（76.2%）。樟脑（54.3%）是 MDEO 中的主要化合物，其次是 1,8-桉叶素（12.2%）和莰烯（10.4%）。此外，还评估了 MDEO 的细胞毒性、抗菌和抗氧化活性。MDEO 对所有癌细胞系均表现出有趣的细胞毒性活性，IC 值为 83.6 至 91.2 μg/mL，尤其是对 MCF-7 癌细胞。使用 Annexin VFITC 和/或碘化丙啶（PI）染料进行标记和流式细胞仪分析，定量证实了 MCF-7 细胞的凋亡诱导。MDEO 对所有细菌和真菌菌株均表现出相当大的抗菌活性，最低抑菌浓度（MIC）值为 0.07 至 1.25 mg/mL。最敏感的微生物菌株是 （MIC：0.07 mg/mL）。最低杀菌浓度（MBC）或最低抑菌浓度（MFC）值比 MIC 值高一次。此外，MDEO 还具有很强的减少 β-胡萝卜素漂白的活性，总抗氧化值为 72.6%，在 1000 μg/mL 浓度下对 DPPH 自由基有显著的清除活性（78.4%）。
Zotero 插件