Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Eastern Medicine and Surgery, Qarshi University, Lahore 54000, Pakistan.
Medicina (Kaunas). 2019 Jul 12;55(7):369. doi: 10.3390/medicina55070369.
The current study focuses on an eco-friendly and cost-effective method of C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical scavenging and reducing power were 73.8 ± 0.32 µg/mg extract, 71.8 ± 0.73% and 105.4 ± 0.65 µg/mg extract respectively. The synthesized EpNPs were observed to possess high cytotoxicity against HepG2 cancer cell lines with IC (61.3 µg/mL) as compared aqueous extract with IC of (247 µg/mL). EpNPs were found to be biocompatible and have less effect on human erythrocytes. EpNPs exhibited significant antioxidant potentials and exhibited considerable activity against and with Minimum Inhibitory Concentration (MICs) of 11.12 μg/mL and 11.33 μg/mL respectively. Fungal species and were found susceptible to EpNPs. Results of the current study revealed that EpNPs exhibited considerable antibacterial, antifungal and cytotoxic potentials. Aqueous extract possesses significant anti-radical properties and thus can be useful in free radicals induced degenerative disorders.
本研究关注的是一种环保且经济有效的方法，即 C. A. Mey.介导的绿色合成银纳米粒子作为潜在的细胞毒性、抗菌和抗氧化剂。筛选植物水提取物的总酚（TPC）、总类黄酮含量（TFC）、总抗氧化能力（TAC）和 2,2-二苯基-1-苦基肼（DPPH）自由基清除能力。通过铁氰化钾比色法估计总还原能力。用紫外光谱法、傅里叶变换红外光谱（FTIR）、X 射线衍射和扫描电子显微镜对生物合成的纳米粒子（EpNPs）进行了表征。评价 EpNPs 的抗菌、生物相容性和细胞毒性。植物水提取物的初步植物化学分析显示 TFC 为 20.7 ± 0.21 µg/mg 提取物，TPC 为 117.01 ± 0.78 µg/mg 提取物。TAC、DPPH 自由基清除和还原能力分别为 73.8 ± 0.32 µg/mg 提取物、71.8 ± 0.73%和 105.4 ± 0.65 µg/mg 提取物。合成的 EpNPs 对 HepG2 癌细胞系表现出高细胞毒性，IC（61.3 µg/mL）与水提取物的 IC（247 µg/mL）相比。EpNPs 被发现对人红细胞具有生物相容性和较小的影响。EpNPs 表现出较强的抗氧化能力，并对 和 表现出相当的活性，最低抑菌浓度（MICs）分别为 11.12 µg/mL 和 11.33 µg/mL。真菌种 和 被发现对 EpNPs 敏感。本研究结果表明，EpNPs 表现出相当大的抗菌、抗真菌和细胞毒性潜力。水提取物具有显著的抗自由基特性，因此可用于自由基诱导的退行性疾病。
