El-Zayat Mustafa Mohsen, Eraqi Mostafa M, Alrefai Hani, El-Khateeb Ayman Y, Ibrahim Marwan A, Aljohani Hashim M, Aljohani Maher M, Elshaer Moustafa Mohammed
Unit of Genetic Engineering and Biotechnology, Faculty of Science, Mansoura University, Mansoura City 35516, Egypt.
National Research Center, Department of Microbiology and Immunology, Veterinary Research Division, Dokki Giza 12622, Egypt.
Biomolecules. 2021 Mar 22;11(3):470. doi: 10.3390/biom11030470.
The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and tannin contents were reduced in the case of the prepared samples of nanoparticles than the original values in the aqueous extract. The prepared extract of and its selenium and zinc nanoparticles showed high potency as antioxidant agents as a result of the DPPH assay. The samples were assessed as anticancer agents against six tumor cells and a normal lung fibroblast (WI-38) cell line. The selenium nanoparticles of extract revealed very strong cytotoxicity against HePG-2 cells (inhibitory concentration (IC) = 7.56 ± 0.6 µg/mL), HCT-116 cells (IC = 10.02 ± 0.9 µg/mL), and HeLa cells (IC = 9.23 ± 0.8 µg/mL). The samples were evaluated as antimicrobial agents against bacterial and fungal strains. Thus, selenium nanoparticles showed potent activities against Gram-negative strains (, , , and ), Gram-positive strains , and ), and the fungal strain . In conclusion, the preparation of nanoparticles of either selenium or zinc is crucial for improved biological characteristics.
当前的工作旨在使用作为一种珍贵药用植物的水提取物来合成硒和锌纳米颗粒。通过透射电子显微镜（TEM）、zeta电位以及植物化学成分的变化对制备的纳米颗粒进行了表征。因此，与水提取物中的原始值相比，制备的纳米颗粒样品中的酚类、黄酮类和单宁含量有所降低。由于二苯基苦味酰基自由基（DPPH）测定，制备的提取物及其硒和锌纳米颗粒显示出作为抗氧化剂的高效能。对样品作为针对六种肿瘤细胞和一种正常肺成纤维细胞（WI-38）细胞系的抗癌剂进行了评估。提取物的硒纳米颗粒对肝癌细胞系（HePG-2）（抑制浓度（IC）= 7.56 ± 0.6 µg/mL）、人结肠癌细胞系（HCT-116）（IC = 10.02 ± 0.9 µg/mL）和人宫颈癌细胞系（HeLa）（IC = 9.23 ± 0.8 µg/mL）显示出非常强的细胞毒性。对样品作为针对细菌和真菌菌株的抗菌剂进行了评估。因此，硒纳米颗粒对革兰氏阴性菌株（大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和鲍曼不动杆菌）、革兰氏阳性菌株（金黄色葡萄球菌和枯草芽孢杆菌）以及真菌菌株（白色念珠菌）显示出强效活性。总之，制备硒或锌纳米颗粒对于改善生物学特性至关重要。
