College of Life Sciences, Sichuan Agricultural University, Yaan 625014, China.
College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2019 Jul 14;20(14):3455. doi: 10.3390/ijms20143455.
Buckwheat is an important functional food material with high nutritional value. However, it is still a difficult task for the taxonomy studies of wild buckwheat that are only based on morphology. In order to demonstrate the most efficient DNA barcode in the phylogenetic research of buckwheat, promote the investigation of wild buckwheat, and also reveal the phylogenetic relationship between species, and intron were validated here, which previously have been proved to be promising DNA barcode candidates for phylogenetic studies in genera . Meanwhile, intron + and + could distinguish the relationship between species clearly. Combining the results of morphology and molecular markers, we suggested the buckwheat species should be divided into two subgroups, one subgroup consisted of , , and its related wild species, and the other subgroup included other wild buckwheat species. Our results could fulfill molecular markers of taxonomy research in genera , promote wild buckwheat species identification, and assist in the use of wild buckwheat resources in the future. Additionally, the phylogenetic relationship revealed here could provide valuable information for molecular breeding of buckwheat and provide reference for inter-species hybridization.
荞麦是一种营养丰富的重要功能食品原料。然而，仅基于形态学的野生荞麦分类学研究仍然是一项艰巨的任务。为了展示在荞麦系统发育研究中最有效的 DNA 条码，促进对野生荞麦的研究，并揭示种间的系统发育关系，本研究验证了先前被证明是属中系统发育研究有前途的 DNA 条码候选者的 和 ，同时 intron + 和 + 能够清晰地区分种间的关系。结合形态学和分子标记的结果，我们建议将荞麦分为两个亚组，一个亚组由 、 、 及其相关的野生种组成，另一个亚组包括其他野生荞麦种。我们的研究结果可以为属的分类学研究提供分子标记，促进野生荞麦种的鉴定，并有助于未来野生荞麦资源的利用。此外，这里揭示的系统发育关系可为荞麦的分子育种提供有价值的信息，并为种间杂交提供参考。
