Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.
Int J Mol Sci. 2019 Jul 14;20(14):3456. doi: 10.3390/ijms20143456.
Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy in muscular disorders since they exhibit good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source with high myogenic differentiation. In this context, we isolated CD56+CD82+ cells from the extra eyelid tissue of young and aged patients, and tested in vitro myogenic differentiation potential. In the current study, myogenic cells derived from extra eyelid tissue were characterized and compared with immortalized human myogenic cells. We found that myogenic cells derived from extra eyelid tissue proliferated and differentiated myofibers in vitro, and restored DYSTROPHIN or PAX7 expression after transplantation with these cells in mice with Duchenne muscular dystrophy. Thus, human myogenic cells derived from extra eyelid tissue including the orbicularis oculi might be good candidates for stem cell-based therapies for treating muscular diseases.
骨骼肌干细胞（MuSCs）因其具有良好的肌生成再生能力而被提议作为肌肉疾病细胞治疗的合适候选物。然而，为了获得更好的治疗效果，有必要从具有高肌生成分化能力的合适组织来源中分离人 MuSCs。在这种情况下，我们从年轻和老年患者的外眼睑组织中分离出 CD56+CD82+细胞，并测试了其体外肌生成分化潜能。在本研究中，我们对源自外眼睑组织的肌细胞进行了表征，并与永生化的人肌细胞进行了比较。我们发现，源自外眼睑组织的肌细胞在体外增殖并分化为肌纤维，并且在用这些细胞移植后，在患有杜氏肌营养不良症的小鼠中恢复了 DYSTROPHIN 或 PAX7 的表达。因此，源自包括眼轮匝肌在内的外眼睑组织的人肌细胞可能是治疗肌肉疾病的基于干细胞的治疗的良好候选物。
