Sato Takahiko, Yamanaka Yukito, Ueno Morio, Sotozono Chie
International Center for Cell & Gene Therapy, Fujita Health University, Toyoake, Japan.
Department of Anatomy, Fujita Health University, Toyoake, Japan.
Methods Mol Biol. 2023;2640:13-19. doi: 10.1007/978-1-0716-3036-5_2.
Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy to muscular disorders since they exhibit a good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source that possess high myogenic differentiation. In this context, isolated CD56+CD82+ cells from extra eyelid tissues were tested in vitro myogenic differentiation potential. Primary human myogenic cells derived from extra eyelids including orbicularis oculi, might be good candidates for human muscle stem cell-based research.
骨骼肌干细胞（MuSCs）因其具有良好的肌源性再生能力，被认为是治疗肌肉疾病的合适细胞疗法候选者。然而，为了获得更好的治疗效果，有必要从具有高肌源性分化能力的合适组织来源中分离出人MuSCs。在此背景下，对从眼睑外组织分离出的CD56+CD82+细胞进行了体外肌源性分化潜能测试。源自包括眼轮匝肌在内的眼睑外的原代人肌源性细胞，可能是基于人肌肉干细胞研究的良好候选者。
