用于禽腺病毒的 LAMP 实时浊度检测。
LAMP real-time turbidity detection for fowl adenovirus.
机构信息
Poultry Institute, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology; Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, China.
出版信息
BMC Vet Res. 2019 Jul 23;15(1):256. doi: 10.1186/s12917-019-2015-5.
BACKGROUND
Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established.
RESULTS
The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/μL of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR.
CONCLUSION
The proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.
背景
禽腺病毒（FAdV）是一种传染性病原体，可导致禽类出现黄疸、严重贫血、呼吸困难和生殖障碍。自 2015 年以来，禽腺病毒病在中国肉鸡中迅速传播，造成了巨大的经济损失。本研究建立了一种针对禽腺病毒的环介导等温扩增（LAMP）实时浊度法，该方法具有很强的特异性。
结果
该方法特异性检测禽腺病毒 4 型，最低检测限为 75 拷贝/μL 的提取 DNA。对疑似组织样本的阳性检出率为 33.3%（14/42），与实时 PCR 一致。
结论
所提出的 LAMP 方法可通过实时浊度测定快速准确地检测流行的禽腺病毒，从而为禽腺病毒病的预防和控制提供了一个诊断平台。
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