Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel.
J Magn Reson. 2019 Sep;306:102-108. doi: 10.1016/j.jmr.2019.07.038. Epub 2019 Jul 9.
Application of EPR to biological systems includes many techniques and applications. In this short perspective, which dares to look into the future, I focus on pulse EPR, which is my field of expertise. Generally, pulse EPR techniques can be divided into two main groups: (1) hyperfine spectroscopy, which explores electron-nuclear interactions, and (2) pulse-dipolar (PD) EPR spectroscopy, which is based on electron-electron spin interactions. Here I focus on PD-EPR because it has a better chance of becoming a widely applied, easy-to-use table-top method to study the structural and dynamic aspects of bio-molecules. I will briefly introduce this technique, its current state of the art, the challenges it is facing, and finally I will describe futuristic scenarios of low-cost PD-EPR approaches that can cross the diffusion barrier from the core of experts to the bulk of the scientific community.
EPR 在生物系统中的应用包括许多技术和应用。在这篇短视的展望未来的文章中，我专注于脉冲 EPR，这是我的专业领域。一般来说，脉冲 EPR 技术可以分为两大类：（1）精细结构光谱学，它探索电子-核相互作用，（2）脉冲偶极（PD）EPR 光谱学，它基于电子-电子自旋相互作用。在这里，我专注于 PD-EPR，因为它更有可能成为一种广泛应用、易于使用的台式方法，用于研究生物分子的结构和动态方面。我将简要介绍该技术、它的当前现状、它所面临的挑战，最后我将描述低成本 PD-EPR 方法的未来情景，这些方法可以从专家核心跨越扩散障碍，进入科学界的主流。
