Shin Il-Soo, Kim Do-Hee, Jang Eun Young, Kim Hee Young, Yoo Hwa-Seung
College of Korean Medicine, Daejeon University, Daejeon 34520, South Korea.
College of Korean Medicine, Daegu Haany University, Daegu 42158, South Korea.
J Pharmacopuncture. 2019 Jun;22(2):68-74. doi: 10.3831/KPI.2019.22.008. Epub 2019 Jun 30.
Cultivated wild ginseng (cWG), called SanYangSanSam, has been used clinically in patients with chronic fatigue in Korea. Little is known about effects of the ginseng distilled (volatile) components produced during evaporizaiton. Recently, we first identified one major component from cWG distilled extract, panaxydol, by using mass spectrometry. However, functional properties of cWG distilled extract and panaxydol remains elusive. Therefore, the present study evaluated the effect of cWG distilled extract or panaxydol on exercise-induced fatigue in rats.
Fatigue was induced by forced swimming and the immobility time was analyzed in male Sprague-Dawley rats. The animals received intraperitoneally either vehicle, cWG distilled extract, or panaxydol 10 min prior to beginning of the forced swimming test (FST) once daily for 5 days. After the FST on day 5, we also analyzed fatigue-related biochemical levels including blood urea nitrogen (BUN), lactate acid (LAC), and lactate dehydrogenase (LDH) in serum and levels of glycogen in liver and soleus muscle.
The forced swimming time in cWG distilled extract (0.6 mL/kg)-treated group was significantly longer than that of control group on day 4 and 5. Panaxydol (0.1 and 0.25 mg/kg)-treated groups showed significantly enhanced performance in the forced swimming, compared to control. In addition, a significant decrease in serum LDH level was found in panaxydol-treated group, while there were no alternations in levels of serum BUN and LAC and glycogen in liver or soleus muscle.
The present study demonstrated cWG distilled extract and its active component panaxydol have a function of anti-fatigue.
栽培野山参（cWG），又称三桠三蔘，在韩国已被临床用于慢性疲劳患者。关于蒸发过程中产生的人参蒸馏（挥发性）成分的作用知之甚少。最近，我们首次通过质谱法从cWG蒸馏提取物中鉴定出一种主要成分——人参二醇。然而，cWG蒸馏提取物和人参二醇的功能特性仍不明确。因此，本研究评估了cWG蒸馏提取物或人参二醇对大鼠运动性疲劳的影响。
通过强迫游泳诱导雄性Sprague-Dawley大鼠疲劳，并分析其不动时间。在强迫游泳试验（FST）开始前10分钟，动物每天腹腔注射一次溶剂、cWG蒸馏提取物或人参二醇，持续5天。在第5天的FST后，我们还分析了与疲劳相关的生化指标，包括血清中的血尿素氮（BUN）、乳酸（LAC）和乳酸脱氢酶（LDH），以及肝脏和比目鱼肌中的糖原水平。
在第4天和第5天，cWG蒸馏提取物（0.6 mL/kg）处理组的强迫游泳时间显著长于对照组。与人参二醇（0.1和0.25 mg/kg）处理组相比，对照组在强迫游泳中的表现显著增强。此外，人参二醇处理组的血清LDH水平显著降低，而血清BUN、LAC水平以及肝脏或比目鱼肌中的糖原水平没有变化。
本研究表明，cWG蒸馏提取物及其活性成分人参二醇具有抗疲劳功能。
