School of Food Science and Engineering, Hefei University of Technology, Hefei, P. R. China.
Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei, P. R. China.
J Sci Food Agric. 2019 Feb;99(3):1405-1412. doi: 10.1002/jsfa.9318. Epub 2018 Oct 8.
Macamides, the main active components contained in maca, have attracted increasing attention due to their various bioactivities. In this study, crude macamide extract (CME) and purified macamide extract (PME) were prepared by enzyme-assisted extraction and macroporous resin separation, and the anti-fatigue effects of CME and PME were evaluated in a forced swimming model.
The composition analysis results revealed that both CME and PME mainly contain eight kinds of macamide. Based on the results of a weight-loaded forced swimming test, compared with a control group, CME and and PME groups could prolong exhaustive swimming time, increase levels of liver glycogen (LG) and muscle glycogen (MG), accelerate fatty acid oxidation in serum to provide energy, eliminate the accumulation of blood lactic acid (BLA) and blood urea nitrogen (BUN), and decrease the serum biomarkers for muscle damage, such as lactate dehydrogenase (LDH) and creatine kinase (CK). Histological analysis also indicated that CME and PME attenuated damage to skeletal muscle and the myocardium in mice during exercise.
Two macamide extracts have a beneficial effect on relieving physical fatigue by attenuating the damage of skeletal muscle and myocardium during exercise, and a better effect was observed in the PME group. © 2018 Society of Chemical Industry.
玛咖中的主要活性成分玛咖酰胺，由于其多种生物活性而受到越来越多的关注。本研究采用酶辅助提取和大孔树脂分离法制备了粗玛咖酰胺提取物（CME）和纯化玛咖酰胺提取物（PME），并在力竭游泳模型中评价了 CME 和 PME 的抗疲劳作用。
组成分析结果表明，CME 和 PME 均主要含有 8 种玛咖酰胺。基于负重力竭游泳试验的结果，与对照组相比，CME 和 PME 组可延长力竭游泳时间，增加肝糖原（LG）和肌糖原（MG）水平，加速血清中脂肪酸氧化供能，消除血乳酸（BLA）和血尿素氮（BUN）的积累，降低运动引起的肌肉损伤的血清标志物，如乳酸脱氢酶（LDH）和肌酸激酶（CK）。组织学分析还表明，CME 和 PME 减轻了运动过程中骨骼肌和心肌的损伤。
两种玛咖酰胺提取物通过减轻运动过程中骨骼肌和心肌的损伤对缓解体力疲劳具有有益作用，PME 组的效果更好。 © 2018 英国化学学会。
